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Biotechnology book from C.H.I.P.S.

G Protein-Coupled Receptor-Protein Interactions
edited by Susan R. George
and Brian F. O'Dowd

G Protein-Coupled Receptor-Protein Interactions elucidates how GPCRs organize signal transduction and control intracellular activities.

G Protein-Coupled Receptor-Protein Interactions offers state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.

Contents:

  1. Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies
  2. Cooperativity in Ligand Binding to Deduce Receptor/Receptor Interaction
  3. Analysis of Receptor-Receptor Interaction Using Time-Resolved Fluorescence Resonance Energy Transfer
  4. Real Time Imaging of GPCR-medicated Arresting Translocation as a Strategy to Evaluate Receptor-Protein Interactions
  5. Localization of G Protein Coupled Receptors and Interacting Proteins in Membrane Microdomains
  6. Identification of Peptide Ligand Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling
  7. Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled Receptor-G Protein Complexes
  8. Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Cross-Linking Strategies to Analyze Receptor-Receptor Interactions
  9. Co-Immunoprecipitation as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions
  10. Use of MALDI-TOF and Mass Spectrometry in the Evaluation of Receptor-Protein Interactions
  11. Use of the Yeast Two-Hybrid System to Detect Receptor-Protein Interactions
  12. GST Fusion Protein and Pull-down Strategies to Evaluate Receptor-Receptor or Receptor-Protein Interactions
  13. G-Protein-Coupled Fusion to Monitor Integral Membrane Protein-Protein Interactions
  14. Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G-Protein-Coupled Receptors
  15. Electrophysiological Characterization of GPCR and Ligand-Gated Channel Crosstalk

Index

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G Protein-Coupled Receptor-Protein Interactions
edited by Susan R. George and Brian F. O'Dowd
2005 329 pages $169.00 + shipping
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