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Industrial Chemistry Book from C.H.I.P.S.

Identification of Microorganisms by Mass Spectrometry
edited by Charles L. Wilkins

Identification of Microorganisms by Mass Spectrometry is a multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis.

Features:

  • Metastable atom bombardment pyrolysis mass spectrometry
  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI)
  • MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria
  • High-resolution Fourier transform mass spectrometry (FTMS)
  • Electrospray ionization (ESI) mass spectrometry

Contents

Cultural, Serological and Genetic Methods for Identification of Bacteria

  • Identification of bacteria by cultural methods
  • Identification of bacteria by serological methods
  • Identification of bacteria by genetic methods
  • Other methods used for bacterial characterization

Mass Spectrometry: Identification and Biodetection, Lessons Learned and Future Developments

  • Analysis of Fatty Acid and Sugar Monomers using GC-FID, GC-MS and GC-MS-MS
  • Analysis of PCR products using PCR, PCR-MS and PCR-MS-MS
  • Analysis of proteins using MALDI-TOF MS
  • Chemical markers for protein-based identification or biodetection

An Introduction to Maldi TOF MS Analysis of Whole Bacteria

  • Mass Spectrometry and Time-of-Flight MS
  • Matrix Assisted Laser Desorption Ionization
  • MALDI/TOF Mass Spectrometry
  • MALDI TOF and Bacterial Identification

The Development of the Block II Chemical Biological Mass Spectrometer

  • Development History and Design Philosophy
  • Requirements and Specifications
  • Performance Testing

Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry

  • Sample Preparation for Rapid, Reproducible Cell Culture
  • Analytical instrumentation for sensitive detection and spectral reproducibility
  • Spectral library assembly
  • Pattern Recognition Methods for Objectively Classifying Bacteria

MALDI TOF Mass Spectrometry of Intact Bacteria

  • MALDI MS of Cellular Extracts
  • Taxonomy: From Isolates to Whole Cell MALDI
  • Whole Cell MALDI MS
  • Biology Based Changes in Whole Cell MALDI Spectra
  • Analysis of Mixtures
  • Experimental Approaches
  • Identification of Protein Markers
  • Analysis of Target Proteins
  • Analysis of Clinical Isolates

Development of Spectral Pattern Matching Approaches to Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Bacterial Identification

  • MALDI-MS Signature Library Construction and Identification

Studies of Malaria by Mass Spectrometry

  • Plasmodium in Red Blood Cells
  • Experimental Protocols for LDMS Detection of Malaria
  • Malaria Detection by Laser Desorption Mass Spectrometry
  • MS-Based Proteomics of the Plasmodium Parasite

Bacterial Strain Differentiation by Mass Spectrometry

  • Analysis of Cellular Proteins by Mass Spectrometry
  • Application of MALDI-TOF to Bacteria Identification

Bacterial Protein Biomarker Discovery: A Focused Approach to Developing Molecular Based Identification Systems

  • Protein Extraction Methods
  • Mass Spectrometry
  • Automating the Process
  • Collecting and Sequencing Proteins

High Throughput Microbial Characterisations Using Electrospray Ionisation Mass Spectrometry and Its Role in Functional Genomics

  • Microbial characterisations beyond the genomic level: Functional genomics
  • Electrospray (ionization) mass spectrometry (ESMS)
  • ESMS of Microbes
  • Direct infusion ESMS of crude cell extracts for high-throughput characterizations - metabolic fingerprinting and footprinting

Bioinformatics for Flexibility, Reliability and Mixture Analysis of Intact Microorganisms

  • Library Matching
  • Machine Learning
  • Bioinformatics
  • Protein Molecular Masses
  • Protein Maps
  • Microsequences from Peptides and Proteins

MALDI-FTMS of Whole Cell Bacteria

  • Fundamentals of MALDI-FTMS
  • Fundmentals of Complex Biological Analysis
  • Whole Cell Characterization Through MALDI-FTMS
  • Recombinant Over-Expressed Proteins Desorbed Directly from Whole Cells

A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole Cell Bacteria by MALDI-TOF-MS

  • Bacterial Identification
  • Immunocapture of Bacterial Mixtures
  • Bacteriophage Amplification of Bacteria

Discrimination and Identification of Microorganisms by Ppyrolysis Mass Spectrometry: From Burning Ambitions to Cooling Embers - A Historical Perspective

  • Introduction to microbial characterisation
  • Principles of PyMS
  • Early developments and investigations (1952 to 1985)
  • The mid 1980s and beyond
  • The move from cluster analyses to neural networks
  • Reproducibility of PyMS

Index

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Identification of Microorganisms by Mass Spectrometry
edited by Charles L. Wilkins
2005 352 pages $99.00 + shipping
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