Pharmaceutical Biotechnology explores the development of protein-based therapeutic substances.
Features:
- Provides a working knowledge of the preparation, stability, and formulation of protein and peptide drugs
- Examines the successful evolution of humanized monoclonal antibodies (MAbs) to treat rheumatoid arthritis and other diseases
- Considers the currently disappointing results of genetic engineering and genomics that have isolated disease-responsible genes without significant clinical effect to date
- Looks at the market and scientific reasons behind the failure of many biotechnology products to live up to their hype
- Discusses regulatory and compendial issues and concludes with a look at future challenges facing biotechnology
Contents
Proteins
- Protein Structure
- Protein Biosynthesis
- Protein Modification
- Protein Stability
Recombinant DNA Basics
- The Uses of Recombinant DNA
- Gel Electrophoresis
- Nucleic Acid Hybridization Assays
- The Polymerase Chain Reaction
- Constructing Recombinant DNA Molecules
- Engineering Protein Sequences
- Examples of Applied Recombinant DNA Technology
Monoclonal Antibodies
- What are Monoclonal Antibodies?
- Applications of Monoclonal Antibodies
- The Generation of Monoclonal Antibodies
- Genetic Engineering
- Production Methods
- Pharmaceutical, Regulatory, and Commercial Aspects
Proteomics: A New Emerging Area of Biotechnology
- What is a Proteome
- Technologies for Proteomics
- Protein Identification
- Bioinformatics
- DNA and Protein Microarray Technologies
- Pharmaceutical and Medical Applications of Proteomics
Commonly Used Analytical Techniques for Biotechnology Products
- Analytical Tools for Proteins
- Spectroscopic and Photometric Methods
- Chromatographic Methods
- Electrophoresis
- Bioassays
Formulation of Proteins and Peptides
- Making Small Protein Particles: Precipitation of Proteins from Supercritical Fluids
- Parenteral Drug Delivery Systems
- Drug Delivery through the Skin
- Multiphase Drug Delivery Systems
- Protein Compaction
- Self-Emulsifying Drug Delivery Systems
- Inclusion Compounds and Cyclodextrins as Delivery Systems
Proteins as Drug Delivery Systems
- Collagen
- Gelatin
- Albumin Microparticles
Proteins and Phospholipids
- Structural Properties of Phospholipids
- Injectable Lipid Emulsions
- Formulation and Preparation
- The Physical State of Phospholipid-Stabilized Emulsions
- Peptides and Phospholipids
- Cochleal Phospholipids Structures
Pulmonary Drug Delivery Systems for Biomacromolecules
- The Pulmonary Route of Drug Delivery
- Anatomy and Physiology of the Respiratory System
- Lipid-Based Pulmonary Delivery
- Solid Colloidal Particles
- Poly(dl-lactide-co-glycolide)
- Polycyanoacrylates
- Gelatin
- Poly(ether-anhydrides)
- Diketopiperazine Derivatives
- Polyethyleneimine (PEI)-DNA Complexes
- Poly(ethylene glycol) Conjugates
- Factors Affecting Pulmonary Dosing
Polymeric Systems for Oral Proten and Peptide Delivery
- Polymers Used for Controlled Drug Delivery
Vaccines: Ancient Medicines to Modern Therapeutics
- Requirements of an Ideal Vaccine for Today
- Types of Modern Vaccines
- Emerging Vaccine Types
- The Immune System and Mechanisms of Action
- Types of Immune Defense Mechanisms
- The Mucosal System
- Vaccine Adjuvants
- Modern Microparticulate Vaccine Vehicles
- The Future of Vaccines and Vaccination
Gene Therapy: An Overview of Current Viral and Non-Viral Vectors
Regulatory and Compendial Issues
- The FDA
- The United States Pharmacopeia
Some Challenges Relating to the Future of Biopharmaceutical Technology
- Examples of Essential Biopharmaceuticals
- Other Expensive Drug Therapies
- Pharmacoeconomics and Value for Money
- Generic Biotechnological Drug Issues
- Alternatives to Protein Biologics
- Gene Engineering
Index