Contents

1. Methods and Application of Anticancer Bioassays

• MTT Assay for Six Carcinoma Cells
• Flow Cytometric Assay for Cell Apoptosis
• DNA Fragmentation Assay
• Bcl-XL/BH3 Interaction Assay
• Dissociation Enhanced Lanthanide Fluoro-immuno Assay (DELFIA)
• Ishikawa Cell and Rat Assay for Detecting Antiestrogens
• ATP Assay for Eight Cells
• Alkaline Phosphatase Activity Assay
• Tumor Endothelial Cell Tube Formation Assay
• Anti-Angiogenic Assay
• In Vivo Hollow Fiber Assay
• VX2 Rabbit Lung Assay
• Insulin-Like Growth Factor-I Induced Kinase Receptor Activation Assay
• Insulin-Like gD·trkA Induced Kinase Receptor Activation Assay
• UV Spectra Based Calf Thymus DNA Intercalation Assay
• Fluorescence Spectra Based Calf Thymus DNA Intercalation Assay
• P-Glycoprotein Pump in MCF-7R Cells Assay
• P-Glycoprotein Pump Related Efflux Carriers Assay
• [³H]Substrate Transport Inhibition Assay
• Lactate Dehydrogenase Release Assay
• Functional Assay of Mitochondrial P-gp
• Resistance Index Value Assay

• Nonradioactive HIV-1 Reverse Transcriptase Activity Assay
• Respiratory Syncytial Virus Assay
• Influenza Virus Types A and B Assay
• Nasal Exhaled NO Concentration Assay
• Nasal NOS2 mRNA Quantity Assay
• RT-PCR and Swine Assay for Anti-HEV Antibody
• HIV-1 Protease and Reverse Transcriptase Kinetic Assay
• Anti-HIV Assay
• Robust Antiviral Assays
• HIV/SIV Fusion Assay
• Rapid DNA Hybridization Assay
• Antiviral Screening Assay for HepAD38 Cell Cultures
• Trak-CTM HCV Core Assay

• Mycobacterium tuberculosis Assay
• DNA Polymerase β Lyase Assay
• Agar Dilution Assay for In Vitro Antitubercular Activity
• Microplate Alamar Blue Assay for In Vitro Antitubercular Activity
• Radiometric Respiratory Assay for In Vitro Antitubercular Activity
• Mycobacterium bovis BCG Inhibition Assay

• In Vitro Anti-Platelet Aggregation Assay
• Fibrinolytic Area Assay
• TXB2 and PGD2 TLC Assay
• TXA2 Synthase Activity Assay
• [Ca2+]i Measuring Assay
• Arachidonic Acid Liberation Assay
• Serotonin Secretion Assay
• cAMP Release Assay
• Ex Vivo Anti-Platelet Aggregation Assay for Patients
• ATP Release Assay
• PAF-Induced Mice Mortality Assay
• PGE2 and TXB2 ELISA
• Thrombelastograph Assay
• Image Monitored FeCl3-Induced Thrombosis Assay for Rat
• Weight Monitored FeCl3-Induced Thrombosis Assay for Rats
• Occlusion Time Monitored FeCl3-Induced Thrombosis Assay for Pig
• Doppler Blood Flow Monitored FeCl3-Induced Thrombosis Assay for Mouse
• Rat Groin Flap Assay
• Ferret Acute Thrombosis Assay
• Rat Acute Thrombosis Assay
• Arterio-Vermus Shunt Assay
• Plasma Clotting Time Assay
• Thromboembolic Photochemical Assay for Repeated Stroke in Mice
• Euglobulin Clot Lysis Assay
• Clot Formation and Lysis (CloFAL) Assay
• Fibrin Microplate Assay
• Fibrinolytic Activity Assay
• Fibrinolysis Assay
• Thrombolytic Assay

• Ecarin Chromogenic Assay
• Anticoagulation Activity Assay in An In Vitro System
• Anticoagulation Activity Assays in An In Vivo System
• Rat Thrombosis Assay
• In Vivo Microvascular IVC Blood Flow Assay
• Owren PT Assay
• Rabbit Double-Balloon Injury Assay
• A Rapid Point of Care Assay for Enoxaparin
• Thromboplastin Clotting Assay
• Rat Assay for Reproducible Stasis-Induced Venous Thrombosis
• In Vivo ACT II/Ecarin Clotting Time Assay
• Ex Vivo and In Vivo Anticoagulation Assay
• Plasma-Based Ecarin Clotting Time Assay for r-Hirudin
• Protamine Titration for Heparin in Whole Blood
• Automated Assay Evaluating Response of Kaolin ACT to Heparin
• Platelet/Monocyte Interaction Based PM Exposure Mouse Assay
• Mouse Tail-Bleeding Time Assay
• MRI Assay for Rabbit Atherosclerotic Lesions
• Spiral Computed Tomography Assay
• Duplex Ultrasound Assay
• Standard Hemochron Assay
• Platelet Serotonin Release Assay
• Activated Partial Thromboplastin Time (APTT) Assay
• Rat Stroke Outcome Assay

• Human Plasma New Pressor Protein (NPP) Assay
• Pulmonary Hypertension Assay
• Coronary Arteries (CA) Constriction Assay
• MRI and Brain Natriuretic Peptide Assays
• Competition Enzyme-Linked Immunosorbent Assay
• Right Ventricular Pressure Assay
• Plasma Nitrite/Nitrate Concentration Assay
• Adeno-Associated Virus Vector Caused Rat Pulmonary Artery Pressure Assay
• Adeno-Associated Virus Vector Caused Rat Protein and mRNA Assay
• N-Terminal pro-Brain Natriuretic Peptide (N-T proBNP) Assay
• Middle Cerebral Artery Occlusion (MCAO) Assay
• Vascular Endothelial Growth Factor (VEGF) Level Assay
• Antispasmodic Agent In Vivo Action Assay
• Temperature Assay in Awake Subjects

• Flow Cytometric Assay for Cellular DNA Content and Caspase-3
• Swine Resuscitation Assay
• HTLV-tax1 or pMuLV-SV-nlslacZ Vectors Transfected Cell Assay
• Parkinsonian Rat Assay
• ELISA for Nerve Growth Factor (NGF) Antigen
• Assay for β-Nerve Growth Factor Levels in Cerebrospinai Fluid
• MDCK Scatter Assay
• Facial Nerve and Spinal Root Avulsions
• TRAb Assays
• Human Thyrotropin Receptor (hTSHR) Assay
• Soluble ICAM-1, TSAb and TBIAb Activity Assays
• Microarray Immunoassay for hTSHr Production
• Affinity Assay for [35S]GTP?S Binding to Gas/olf
• Tissue Segment Binding Assay for α1B-Adrenoceptor
• Membrane Binding Assay with Rat Cerebral Cortex for α1B-Adrenoceptor
• Whole Cell Binding Assay for α1B-Adrenoceptor
• Functional Assay for α1A,B-Adrenoceptor
• Rat Neuroprotective Assay
• GABA-Benzodiazepine Receptor Assay
• Forced Swimming and Tail Suspension Assays
• IGF-I Kinase Receptor Activation (KIRA) Assay
• gD.trkA Kinase Receptor Activation (KIRA) Assay
• gD.trk KIRA-ELISA
• Assays for Cannabinoid Receptors in Rat Cerebella or Mouse Brains

• Assay for Oxidative Stress in Cerebral Cortex of AD Mice
• Reporter Assay for Primary Neuronal Cultures
• Electrophoretic Mobility Shift Assay (EMSA)
• Binding Assays Using Aggregated Aβ peptide in Solution
• Assay for Muscarinic Receptor 1 in Alzheimer’s Dementia Model
• β-Secretase Activity Assay
• Aβ Fibril Binding Assay
• TLC and Microplate Assays for Acetylcholinesterase Inhibitors
• Immunocapture Assay Measuring Specific Enzyme Activity of Neprilysin
• In Vivo AChE Inhibition Assay
• Single Particle Assay for Aβ Aggregates
• Indirect Immunofluorescence Assay
• Mouse Behavioral Assays
• Center of Pressure (CoP) Assay in Mice
• Assay for Plasma Levels of DJ-1
• Membrane Filter Assay for Tau Aggregation
• Assays for Motor Neuron Degeneration
• HPLC Assay for Neuroprotective Agent in Mice Plasma
• Tissue Culture Assays for SOD1 Mutations
• Luciferase-Based Reporter Assay

• Rat Bone Mineral Density Assay
• Osteoblastic Cell Proliferation and Alkaline Phosphatase (ALP) Activity Assays
• Murine Osteoblastic MC3T3-E1 Cell Calcification and Van Kossa Assays
• Osteoclast Generation Assay for Male Senile Rat
• Bone Resorption and Recovery Related Assays
• Mouse Bone Mineral Density Assay
• PPAR-γ Competitor Assay
• Human Serum Estrogen Level Assay
• Luciferase Activity Assay
• IL-1β and TNF-α Level Assay
• ER Binding and Receptor Activity Assays
• Fluorescent Estrogen Receptor Assay
• ELISA for Urinary Helical Peptide
• Urine Midmolecule Osteocalcin Assay
• BMD and Osteocalcin Assay

• Rat Mast Cell Histamine-Release Assay
• Rabbit Aortic Force Assay
• Dopaminergic Cell Death Based Neural Transplantation Assay
• Mast Cell Degranulation Assay
• Basophils Assay as Allergen
• RBL-2H3 Cell Desensitization Assay
• Migration Assay of Dendritic Cell from PBMCs
• Mouse EAE Induction Assay
• COSTIM Assay for DC/T-Cells
• Cytokine Assay for L-6, IL-10, IL-12, and TNF-α of DCs
• ELISPOT Assay for DCs’ IFN-g
• DC Function Assay for Evaluating Toll-like Receptor Function
• Migration Assay of Dendritic Cell from Bone Marrow of A/J Mice
• Lymphoid Organ Assay
• ELISA of IFN-γ from Human Myelomonocytic KG-1 Cells
• Human Whole Blood IFN- γ Assays
• Sheep Whole Blood IFN- γ Assays
• ELISPOT Assay for IFN- γ
• IFN-β RG Assay
• Anti-rHuEPO NAb Assay
• Chloramphenicol Acetyltransferase Assay
• Chemotaxis Assay
• Fibroblast-Populated Microsphere Assay
• Fibroblast-Populated Concentric Microsphere Assay
• Radial Assay of Chemotaxis
• Antibody Forming Cell Assay
• Immunosuppressive Assay
• Cell Based ELISA
• Large Animal Lung Transplantation Assay

• Adhesion Formation Assay
• Ligand Complex Based Adhesion Assay
• Human Umbilical Vein Endothelial Cell Assay
• Pleurisy Mouse Assay
• Proliferation of Peripheral Blood Mononuclear Cell Assay
• COX-1, COX-2 and 5-LOX Assay with [1-14C]Arachidonic Acid
• COX-1 and COX-2 Assay with Human Whole Blood
• o-Hydroxyleukotriene B4 Assay
• Rolling and Adherent of Leukocytes Assay
• CCR5 Receptor Binding Assay
• Tissue Binding Affinity Assay
• G93A-SOD1 Transgenic Mouse Assay
• MCP-1 Induced ERK1 and ERK2 Phosphorylation Assay
• LPS and IL-6 Induced ERK1 and ERK2 Phosphorylation Assay
• ELA4.NOB-1/CTLL Cell Assay
• FK506 Binding Protein 51 (FKBP51) mRNA Assay
• Xylene-Induced Ear Edema Assay

• Blood and Plasma Total Antioxidant Capacity (TAC) Assay
• Ferric Reducing-Antioxidant Power (FRAP) Assay
• Human LDL Oxidation Assay
• DPPH Radical Cation Scavenging Assay
• ABTS+ Radical Cation Scavenging Assay
• Lipid Peroxidation Assay Using Rat Brain Tissue
• Flow Through Chemiluminescence (FTCL) Assay
• Superoxide Radical Scavenging Assay
• Deoxyribose Assay for Hydroxyl Radical Scavenging Activity
• DNA Nicking Assay for Hydroxyl Radical Scavenging Activity
• Oxidative Lag-Time Assay
• TBARS and Electrophoresis Assay
• Reporter and Electrophoretic Mobility Shift Assay (EMSA).
• [Ca2+]cyt Assay
• Quantitative Real-Time PCR Assay
• FT-IR Based Assay for Antioxidation Activity of Ionol and Piperidone
• HPLC Assay for Anti-oxidation Potential of Polyphenol
• ROS Production Assay
• Rabbit LDL Oxidation Assay
• ROS Scaverging Assays
• DNA Damage Assay
• Egg Yolk Thiobarbituric Acid Reactive Substances (TBARS) Assay
• Mouse Catalase (CAT) Assay
• Rat Tissue Thiobarbituric Acid Reactive Substances (TBARS) Assay
• Antioxidant Activity Assay for β-Carotene/Linoleic Acid System
• Rat Brain Tissue NO Assay
• Rat Brain Antioxidative Enzyme Assay
• Rat Brain Hippocampi Protein Oxidation Assay

• Algogenic Activity Assay for Rat Ureteral Stone
• Cold Pressor Based Assay for Acute Pain of Healthy Volunteers
• Heat Based Assay for Acute Pain of Healthy Volunteers
• Electrical Stimulation Based Assay for Acute Pain of Healthy Volunteers
• Radiant Heat Tail-Flick Assay for Mice
• Pain Behaviors/Responses Assays in Rats
• Hot Plate Assay in Rats
• Plantar Assay in Rats
• Hot Plate Assay in Mice
• Paw and Tail Formalin Assays in Mice
• Rat Assays for Bone Cancer Pain
• Mouse Assay for Hind Paw Cancer Pain
• Visceral Pain Assay
• Canine Nociceptive Thermal Escape Assay in Dog
• Carrageenan Assay in Rats
• Electrophysiological Assay for Mice with Tumor-Evoked Hyperalgesia
• Mouse Assay for Bone Cancer Pain

• Handling-Induced Seizure Susceptibility (HISS) Assay
• Mouse Locomotor Activity Assay
• Mouse Diathesis-Stress Assay
• Social Interaction Assay
• Spatial Learning Ability Assay for Recurrent Seizure Rats
• Timed Pentylenetetrazol (PTZ) Infusion Assay for Mice
• PTZ Seizure Assay for Mice
• Maximal Electroconvulsions Threshold Assay for Mice
• Maximal Electroshock Seizure (MES) Assay
• 6Hz Psychomotor Seizure Assay for Mice
• Subcutaneous Bicuculline and Picrotoxin Assay for Mice
• N-Methyl-D-aspartate (NMDA)-Induced Convulsions Assay for Mice
• Audiogenic Seizure (AGS) Assay
• Kindled Rat Assay for Focal Seizures
• Cobalt/Homocysteine Assay for Status Epilepticus of Rats
• PTZ-Induced Kindling Assay
• Increasing-Current Electroshock Seizure (ICES) Assay for Mice

• Islet Xenograft Assay for Diabetic Mice
• Assays for Spontaneous Diabetes and Adoptive Transfer of Diabetes
• Oral Glucose Tolerance Assays for Patients
• Injection Glucose Tolerance Assays for Rats
• Renal Cortical TGF-β1 Protein Assays for Rats
• Low Dose Streptozotocin Treated Heminephre Ctomized Rat Assay
• Rat Early Diabetic Nephropathy Assay
• Urinary Endothelin-1 Excretion Assay for Type II Diabetes Rats
• Subtotally Nephrectomized Rat Assay
• Type II Diabetes Mice Assay

• Colorimetric Yeast Assay for Trichothecene Mycotoxins
• Colorimetric Cell Proliferation Assay
• Yeast DEL Assay
• NCCLS and EUCAST Assays
• Alcohol Dehydrogenase Based Colorimetric Assays
• Colorimetric Assay for Iron in Yeast
• Mcroplate Redox Assays of E. Coli
• Ciliate Tetrahymena Thermophila Assay for Trichothecene Mycotoxins
• Fluorescent Dyes Based Cell Viability Assay for Triton X-100 Toxicity
• MTT Assay for Fusarium Mycotoxins
• Mortality and Frass Production Assay for Toxicity of Bacterial Strains
• Cell Toxicity Assay
• Dehydrogenase (Dhase) Inhibition Assay
• Sediment Toxicity Assay
• Toxicity Assay of Particle-Associated Arsenite and Mercury
• Genetic Toxicity Assay
• Microtoxβ Assays
• DNA Piezoelectric Biosensor Assay
• Protein Phosphatase Inhibition Assay and ELISA of Microcystins
• Lepidium Sativum Assay for Microcystin Toxicity
• Antiproliferative Assay for Interleukin-4

• Lux-Fluoro Assay for Combined Genotoxicity and Cytotoxicity of Chemicals
• Flow Cytometry and Microscopy Based Assay
• Cell Transformation Assay
• ALIC-Based Assay for Toxicity of Chemicals in SPM
• Immunization in the Murine Assay
• Immunotoxicological Functional Assay
• PWM-Induced IgM Assay for Toxicity of Chemicals
• Tetrachlorodibenzo-p-dioxin (TCDD)-Induced Toxicity Assay
• H4IIE EROD Assay
• Rat Mutation Assay
• Ah Receptor and Related Assay
• Ectonucleotidase Expression Assay
• Toxicity Equivalent Quantity (TEQ) Assay
• Enzyme Lacking Arachidonic Acid Epoxygenase Activity Assay
• Japanese Medaka Embryo-Larval Assay
• Green Fluorescent Protein-Based Cell Assay
• RACB Assay for Ovarian Toxicity of Xenobiotics
• Uroepithelial Cell Assay
• Male Rat Systemic Toxicity Assay
• 28 Day Oral Toxicity Assay
• Endocrine Disruptors Assay
• Mating Efficiency Assay
• Soils Assay for Contaminants and Toxicity
• Rice Nitrate Reductase Activity Assay
• Tradescantia-Micronucleus Assay
• Nitric Oxide Neurotoxicity Assay
• EBV-Transformed Human Burkitt’s Lymphoma Cell Assay
• Murine Bone Marrow Assay for Haemopoietic and Osteogenic Toxicity
• Pnar-gfp Assay for Carcinogenic Toxicity of Nitrate
• Brine Shrimp (Artemia Salina) Assay
• DPPH Radical Scavenging Property Assay

• GST-P Enzyme-Altered Foci Assay
• GST-P+ and TGF-α+ Foci Assay
• Partial Hepatectomy Assay
• In Vivo Short-Term Liver Initiation Assay in Rats
• TUNEL Related Aassay
• Haematopoietic Progenitor Cells Mobilization Assay
• Electrophoretic Mobility Shift Assay
• Telomerase Activity Assay
• Von Willebrand Factor (vWf) Level Assay
• Bromodeoxyuridine Incorporation Assay
• NF-kB Activation Assay
• Rat Summation of Initiation Activity Assay
• Hepatoprotective Assay
• Cytokine Gene Expression Assay
• Competitive Inhibition Assay
• Marine Toxin Okadaic Acid (OA) Assay
• Antibody-Based Assays for Omega-Conotoxin MVIIA
• Rituximab Assays
• Immunological Assay
• Proliferation Assay for of Bovine Capillary Endothelial Cells
• Body Weight Based Liver Tumor Incidence Assay

• Yeast Oestrogen Assay
• In Vivo, Ex Vivo and In Vitro Assays for Estrogen-Like Effect
• Ameliorative Yeast Assay
• In Yeast Two-Hybrid Assay
• Estrogen-Sensitive Yeast Strain RMY/ER-ERE Assay
• Bioluminescent Yeast Assays
• LYES Assay
• ERα and ERβ Stable Transactivation Assay
• HELNα and HELN? Transfected Cell Assay
• Luciferase Reporter Gene Assay
• Target AKT Pathway’s Assay
• Luciferase Assay
• Androgen Receptor Transactivation Assay
• Green Fluorescent Protein Expression Assay
• Luteinising Hormone Releasing Hormone (LHRH) Assay
• Ishikawa Endometrial Cancer Cell Assay for Estrogen Receptor Expression
• Ishikawa Cell Assay for 11?-Hydroxysteroid Dehydrogenase Type 2 Activity
• Ishikawa Cell Assay for Estrogen Activity
• Ishikawa Cell Assay for Plasminogen Activator Inhibitor-1 (PAI-1)
• Testosterone Induction Assay with Sertoli Cell
• Electrophoretic Mobility and Antibody Shift Assays

• Plasmodium Falciparum and Murine P388 Leukemia Cell Assay
• Antiplasmodial Activity Assay
• Plasmodium Falciparum Growth In Vitro Assay
• Antibody Assay for Red Blood Cell Polymorphisms
• Leishmania Macrophage Assay
• Lactate Dehydrogenase Based Antiplasmodial Assay
• Histidine-Rich Protein II Assay
• Histidine-Rich Protein II Assay
• Antimalarial Activity Assay
• Plasmodium Yoelii Liver Stage Parasites Inhibition Assay
• In Vivo Antimalarial Assay
• β-Hematin Inhibition Assay
• Non-radiolabelled Ferriprotoporphyrin IX Biomineralisation Inhibition Assay
• Survival of Anopheles Gambiae Assay
• Chloroquine Assay
• MS Assay for Quantification of Chloroquine in Dog Plasma
• Sensitive Fluorescence HPLC Assay for AQ-13
• HPLC and HPTLC Assays for Chloroquine, Primaquine and Bulaquine
• Real-Time PCR Based Chloroquine Sensitivity Assay
• Rat Embryos In Vitro Assay
• HPLC-MS Assay for β-DHA in Rat Plasma
• Plasmodium Falciparum Clone and DHA Assay

• UT-7/EPO Cell Proliferation and Neutralizing Anti-EPO Antibody Assays
• Assay System for Biotin Protein Ligase (BPL) from Escherichia Coli
• β-Galactosidase and α-Amylase Biosynthesis Assays
• Penicillin-Binding Protein (PBP 5) and Vancomycin Activity Assays
• Recombinant Bacteria Assay Evaluating Androgen Biosynthesis
• UDP-Glc 4-Epimerase Assay
• ATPs Exchange Assays
• Lux-Fluoro Assay for Combined Genotoxicity and Cytotoxicity
• Reporter Gene Assay for Aryl Hydrocarbon Receptor
• CYP1A Enzyme Assay
• CYP1A Activity (EROD) Assay
• Deubiquitinating Enzyme (DUB) Activity Assay
• Flow Cytometric Assay
• Serum Neutralization Assay Based on rHMPV-GFP
• Yellow Fluorescent Protein-Based Assay
• Red Fluorescent Cytotoxic T Lymphocyte Assay
• Green Fluorescent Protein-Based Assay
• DNA Break Assay in HepG2 Cells