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Industrial Book from C.H.I.P.S.

Practical High-Performance Liquid Chromatography
Fifth edition
by Veronika R. Meyer

Practical High-Perfromance Liquid Chromatography covers:

  • Comparison of HPLC with capillary electrophoresis
  • How to obtain peak capacity
  • van Deemter curves and other coherences
  • Hydrophilic interaction chromatography
  • Method transfer
  • Comprehensive two-dimensional HPLC
  • Fast separations at 1000 bar
  • HPLC with superheated water
  • Is it worth the effort?
  • Verification with a second method
  • Method validation
  • Standard operating procedures
  • Measurement uncertainty
  • Qualifications, instrument test, and system suitability test
  • The quest for quality

Contents

Part 1: Introduction

  • HPLC: A powerful separation method
  • A first HPLC experiment
  • Liquid chromatographic separation modes
  • The HPLC instrument
  • Safety in the HPLC laboratory
  • Comparison between high-performance liquid chromatography and gas chromatography
  • Comparison between high-performance liquid chromatography and capillary electrophoresis
  • Units for pressure, length and viscosity
  • Scientific journals
  • Recommended books

Part 2: Theoretical Principles

  • The chromatographic process
  • Band broadening
  • The chromatogram and its purport
  • Graphical representation of peak pairs with different degree of resolution
  • Factors affecting resolution.
  • Extra-column volumes
  • Tailing
  • Peak capacity and statistical resolution probability
  • Effects of temperature in HPLC
  • The limits of HPLC
  • How to obtain peak capacity

Part 3: Pumps

  • General requirements
  • The short-stroke piston pump
  • Maintenance and repair
  • Other pump designs

Part 4: Preparation of Equipment up to Sample Injection

  • Selection of the mobile phase
  • Preparation of the mobile phase
  • Gradient systems
  • Capillary tubing
  • Fittings
  • Sample injectors
  • Sample solution and sample volume

Part 5: Solvent Properties

  • Table of organic solvents
  • Solvent selectivity
  • Miscibility
  • Buffers
  • Shelf life of mobile phases
  • The mixing cross

Part 6: Detectors

  • General
  • UV detectors
  • Refractive index detectors
  • Fluorescence detectors
  • Electrochemical detectors
  • Light-scattering detectors
  • Other detectors
  • Multiple detection
  • Indirect detection
  • Coupling with spectroscopy

Part 7: Columns and Stationary Phases

  • Columns for HPLC
  • Precolumns
  • General properties of stationary phases
  • Silica
  • Chemically modified silica
  • Styrene-divinylbenzene
  • Some other stationary phases
  • Column care and regeneration

Part 8: HPLC Column Tests

  • Simple tests for HPLC columns
  • Determination of particle size
  • Determination of breakthrough time
  • The test mixture
  • Dimensionless parameters for HPLC column characterization
  • The van Deemter equation from reduced parameters and its use in column diagnosis
  • van Deemter curves and other coherences
  • Diffusion coefficients

Part 9: Adsorption Chromatography: Normal-Phase Chromatography

  • What is adsorption
  • The eluotropic series
  • Selectivity properties of the mobile phase
  • Choice and optimization of the mobile phase
  • Applications

Part 10: Reversed-Phase Chromatography

  • Principle
  • Mobile phases in reversed-phase chromatography
  • Solvent selectivity and strength
  • Stationary phases
  • Method development in reversed-phase chromatography
  • Applications
  • Hydrophobic interaction chromatography

Part 11: Chromatography with Chemically Bonded Phases

  • Introduction
  • Properties of some stationary phases
  • Hydrophilic interaction chromatography

Part 12: Ion-Exchange Chromatography

  • Introduction
  • Principle
  • Properties of ion exchangers
  • Influence of the mobile phase
  • Special possibilities of ion exchange
  • Practical hints
  • Applications

Part 13: Ion-Pair Chromatography

  • Introduction
  • Ion-pair chromatography in practice
  • Applications
  • Appendix: UV detection using ion-pair reagents

Part 14: Ion Chromatography

  • Principle
  • Suppression techniques
  • Phase systems
  • Applications

Part 15: Size-Exclusion Chromatography

  • Principle
  • The calibration chromatogram
  • Molecular mass determination by means of size-exclusion chromatography
  • Coupled size-exclusion columns
  • Phase systems
  • Applications

Part 16: Affinity Chromatography

  • Principle
  • Affinity chromatography as a special case of HPLC
  • Applications

Part 17: Choice of Method

  • The various possibilities
  • Method transfer

Part 18: Solving the Elution Problem

  • The elution problem
  • Solvent gradients
  • Column switching
  • Comprehensive two-dimensional HPLC
  • Optimization of an isocratic chromatogram using four solvents
  • Optimization of the other parameters
  • Mixed stationary phases

Part 19: Analytical HPLC

  • Qualitative analysis
  • Trace analysis
  • Quantitative analysis
  • Recovery
  • Peak-height and peak-area determination for quantitative analysis
  • Integration errors
  • The detection wavelength
  • Derivatization
  • Unexpected peaks: Ghost and system peaks

Part 20: Quality Assurance

  • Is it worth the effort
  • Verification with a second method
  • Method validation
  • Standard operating procedures
  • Measurement uncertainty
  • Qualifications, instrument test and system suitability test
  • The quest for quality

Part 21: Preparative HPLC

  • Problem
  • Preparative HPLC in practice
  • Overloading effects
  • Fraction collection
  • Recycling
  • Displacement chromatography

Part 22: Separation of Enantiomers

  • Introduction
  • Chiral mobile phases
  • Chiral liquid stationary phases
  • Chiral solid stationary phases
  • Indirect separation of enantiomers

Part 23: Special Possibilities

  • Micro, capillary and chip HPLC
  • High-speed and super-speed HPLC
  • Fast separations at 1000 bar: UHPLC
  • HPLC with supercritical mobile phases
  • HPLC with superheated water
  • Electrochromatography

Part 24: Appendix 1: Applied HPLC Theory

Part 25: Appendix 2: How to Perform the Instrument Test

  • Introduction
  • Test sequence
  • Preparations
  • Pump test
  • UV detector test
  • Autosampler test
  • Column oven test
  • Equations and calculations
  • Documentation

Part 26: Appendix 3: Troubleshooting

  • Pressure problems
  • Leak in the pump system
  • Deviating retention times
  • Injection problems
  • Baseline problems
  • Peak shape problems
  • Problems with light-scattering detectors
  • Other causes
  • Instrument test

Index

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Practical High-Performance
Liquid Chromatography

Fifth edition
by Veronika R. Meyer

2010 • 412 pages • $154.00 + shipping
Texas residents please add 6.75 % sales tax

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