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Protein Science Reference from C.H.I.P.S.

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

edited by Gerd Gellissen


While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform.

The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells.

Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example.

Contents

  1. Key and Criteria to the Selection of an Expression Platform
  2. Escherichia coli
  3. Pseudomonas fluorescens
  4. Staphylococcus carnosus and other Gram-positive Bacteria
  5. Arxula adeninivorans
  6. Hansenula polymorpha
  7. Pichia pastoris
  8. Yarrowia lipolytica
  9. Aspergillus sojae
  10. Sordaria macrospora
  11. Mammalian Cells
  12. Plant Cells
  13. Wide-Range Integrative Expression Vectors for Fungi, based on Ribosomal DNA Elements
  14. Comparative Fermentation
  15. Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production
  16. Biopharmaceuticals and the Industrial Environment

Subject Index

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Production of Recombinant Proteins:
Novel Microbial and Eukaryotic Expression Systems
edited by Gerd Gellissen

2005 404 pages $229.00 + shipping

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