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Pharmaceutical Book from C.H.I.P.S.

Producing Biomolecular Substances with Fermentors, Bioreactors, and Biomolecular Synthesizers
by William L. Hochfeld

Producing Biomolecular Substances with Fermentors, Bioreactors, and Biomolecular Synthesizers examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins.

Features:

  • Presents practical techniques, time-proven methods, and essential applications
  • Includes the declassified text of the CIA and Defense Intelligence Agency Report of 28 May, 2003: Iraqi Mobile Biological Warfare Agent Production Plants
  • Delineates what must be done and how to do it effectively, followed by the formula for reaching the necessary value
  • Contains a biotech glossary, numerous references, charts, illustrations, and an extensive index

Contents

Introduction

  • Biotechnology
  • Bioproduction
  • The Bioprocess
  • Cell Lines
  • Cell-Culture Process
  • Separation, Recovery, Purification
  • Virus and Foreign-DNA Removal
  • Quality Assurance

Technology Overview

  • Biomolecular Foundations
  • Cellular Variation
  • Key Molecular Interactions
  • Base-Pair Complementarity
  • Genetic Coding
  • Interrupting Gene Segments
  • Sequence Determinations
  • Cloning
  • Synthesized DNA
  • Cell Lines
  • Expression Systems

Introduction to the Bioprocess

  • Optimizing Product Yield
  • Kinetic Models
  • cGMP Standards
  • Contract Manufacturing
  • Complex Mixtures
  • Biomolecular Products
  • Joint Manufacturing
  • Downscaling to Scale Up
  • Large-Scale Bioprocesses
  • Scaling-Up In-House or in a Contract Facility
  • Lab-to-Pilot-Plant-to-Production
  • Increasing Bioprocess Scale
  • Differing Features at Larger Scale
  • Simulating Environment at Scale
  • Detailed Specifications
  • Impact on Downstream Bioprocessing
  • FDA Biologics Review Process
  • Regulating Biological Products

Recombinant DNA Materials and Methods

  • Cloning
  • Screening and Selection
  • DNA Synthesis
  • Cell Lines
  • Expression Systems
  • Vector Construction
  • Prokaryotic and Eukaryotic Growth Characteristics
  • Expression Levels
  • Intra- vs. Extra-cellular Expression
  • Intracellular Expression
  • Extracellular Expression
  • Glycosylation
  • Mammalian Cell Glycosylation
  • Insect Cell Glycosylation
  • General Considerations
  • Further Post-translational Modifications
  • Economic Concerns
  • Regulatory Concerns
  • Manufacturers' Directory

Enzymes

  • Enzyme Protein Engineering
  • Transformation in Non-Aqueous Systems
  • Restriction Enzymes
  • Restriction-Enzyme Classes
  • Class I Restriction Enzymes
  • Class II Restriction Enzymes
  • Class III Restriction Enzymes
  • Restriction-Enzyme Specificity
  • Star Activity
  • Enzyme Families and Compatible Ends
  • Isoschizomers
  • Reverse Transcriptases
  • DNA and RNA Nucleases
  • Manufacturers' Directory

DNA-Amplification Reagents

  • Amplification by DNA Synthesis (PCR)
  • Amplification by RNA Transcription (TAS and 3SR
  • Amplification by Ligation (LAR, LCR, LAS)
  • Amplification by RNA Replication (Q Replicase)
  • Sequence-Based Amplification
  • Manufacturers Directory

Cell-Culture Media

  • Prokaryotic Cell-Culture Medium
  • Eukaryotic Cell-Culture Medium
  • Defined Medium
  • Serum
  • Other Sera
  • Serum Contamination
  • Serum-Free (Defined) Medium
  • Cell-Culture Gels
  • Media Supplements
  • Attachment Factors
  • Buffers
  • Sodium
  • Earle's Balanced Salt Solution
  • Growth Factors
  • Epidermal Growth Factor (EGF)
  • Fibroblast Growth Factors (FGF)
  • Acidic FGF
  • Basic FGF
  • Other Growth Factors
  • Lectins
  • Toxins
  • Transport Factors
  • Vitamins
  • Water
  • Manufacturers' Directory

Fermenters

  • The Fermentation Process
  • Enzyme Fermentation in the Organic Phase
  • Cell Growth and Production
  • Factors Affecting Specific Growth Rate
  • Kinetic Models
  • Optimal Conditions
  • Recombinant Culture Kinetics
  • Fermenter Design
  • Kinetic Modeling of the Fermentation Process
  • STF Batch Scale Up
  • Power Number Method for STF Geometric Scale Up
  • Continuous-Culture Scale Up
  • Fermenter Manufacturers Directory

Bioreactors

  • Aseptic Conditions
  • Monoclonal Antibody Production
  • mAB Production in Ascites Fluid
  • Bioreactor Engineering and Design
  • Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program
  • Bioreactor Manufacturers Directory

Biomolecular Synthesizers

  • DNA Synthesizers
  • Manufacturers Directory
  • Technology Overview
  • Peptides by Solid-Phase Synthesis
  • Manufacturers Directory

Index

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Producing Biomolecular Substances with Fermentors, Bioreactors, and Biomolecular Synthesizers
by William L. Hochfeld
2006 408 pages $198.95 + shipping
Texas residents please add 6.75 % sales tax

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