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Molecular Biology Handbook from C.H.I.P.S.

Techniques for Molecular Biology
edited by D. Tagu

Techniques for Molecular Biology is presented in the form of profiles, and explains simply the theoretical principles of techniques of molecular biology.

This edition contains new illustrations and describes numerous techniques of genomic studies that have been develo0ped recently in laboratories.

Contents

Definition

  1. Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein
  2. Parameters of Description of a Genome
  3. Sequencing of Entire Genomes

Vectors and Cloning

  1. Restriction Enzymes
  2. Electrophoresis of Nucleic Acids
  3. Description of a Plasmid and a Phagemid
  4. Description of a Bacteriophage and a Cosmid
  5. Description of a YAC and Other Large Vectors
  6. Molecular Cloning
  7. Genetic Transformation of Bacteria and Yeasts

Labelling of Nucleic Acids and Hybridization

  1. DNA Labelling
  2. Molecular Hybridization
  3. In situ Hybridization of mRNA

DNA Libraries and Screening

  1. Construction of a Genomic DNA Library
  2. Construction of a cDNA Library
  3. Screening of a Library
  4. Differential Display: Subtractive Libraries, AFLP-cDNA
  5. Differential Display RT-PCR: mRNA Screening
  6. Differential Display SSH: Suppression Subtractive Hybridization
  7. Differential Screening by RDA: Representational Difference Analysis
  8. EST: Expressed Sequence Tags
  9. DNA Microarrays: DNA Microchips, cDNA Filters

Characterization of a Gene

  1. DNA Sequencing
  2. PCR: Polymerase Chain Reaction
  3. RACE: Rapid Amplification of cDNA Ends
  4. Genome Walking by PCR
  5. RT-PCR: Reverse Transcriptase PCR
  6. In vitro Transcription
  7. Determination of Transcription Initiation Sit
  8. Functional Analysis of Promoters
  9. Gel Retarda-tion
  10. DNAse I Footprinting

Genetic Transformation of Euaryotes

  1. Genetic Transformation of Plants by Agrobacterium tumefaciens
  2. Direct Gene Transfer into Plant Protoplasts
  3. Direct Gene Transfer by Biolistics
  4. Genetic Transformation of Animal Cells
  5. Cloning of Animals
  6. Transient Expression

Analysis of Gene Function

  1. Recombinant Proteins
  2. Baculoviruses of Insects, Vectors of Expression of Foreign Genes
  3. Double Hybrid System
  4. Directed Mutagenesis
  5. Mutation Complementation in Yeast
  6. Knock-out of Genes
  7. Gene Tagging
  8. RNA Interference

Polymorphism of a Genome

  1. Molecular Markers
  2. Genetic and Physical Maps
  3. PFGE: Pulse Field Gel Electrophoresis
  4. RFLP: Restriction Fragment Length Polymorphism
  5. RAPD: Random Amplified Polymorphic DNA
  6. AFLP: Amplified Fragment Length Polymorphism
  7. Retromarkers
  8. SSCP: Single Strand Conformation Polymorphism
  9. DGGE: Denaturing Gel Gradient Electrophoresis
  10. SNP: Single Nucleotide Polymorphism
  11. Simple Sequence Repeats (SSR): Microsatellites

List of Contributors

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Techniques for Molecular Biology
edited by D. Tagu
2006 227 pages $49.95 + shipping

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