Analysis of Growth Factor Signaling in Embryos provides the methods and guidelines for experimental design to study major aspects of cell signaling in vertebrate embryos.

Features:

• Provides practical information for scientists interested in examining signaling pathways in model organisms
• Describes new techniques for studying signaling
• Incorporates molecular, cellular, and systems analysis
• Discusses signal transduction, protein phosphorylation, embryogenesis, pattern formation, and morphogenesis
• Covers multiple types of signaling components, including receptors, GTPases, and effector kinases
• Includes all of the major vertebrate embryonic model systems, such as Xenopus, mouse, zebrafish, and chick

Contents

Signals and Pathways

• Analysis of Canonical Wnt Signaling in Xenopus Embryos
• How to Assay Non-Canonical Wnt Signaling: A Critical Analysis
• Regulation of TGF-ß Family Activity by Proprotein Processing
• Analysis of MAP Kinase Pathways in Vertebrate Development
• Analysis of Retinoid Signalling in Embryos
• Wnt Signaling via the Rho Family of GTPases during Embryonic Development
• Phosphospecific Antibodies as Tools for the Study of Signal Transduction during Development

Ionic Signals

• Dynamic Analysis of Calcium Signaling in Animal Development
• Strategies and Techniques for Investigation of Biophysical Signals in Patterning

Transcriptional Regulation of Target Genes

• Expression Profiling in Xenopus Embryos
• Chromatin Immunoprecipitation for In Vivo Studies of Transcriptional Regulation during Development

Emerging Strategies for the Analysis of Signaling in Development

• Chemical Biology in Zebrafish Vascular Development
• Investigating Gastrulation
• Bringing Small Molecule Regulation of Protein Activity to Developmental Systems
• Systems Analysis of Signaling Pathways

Index

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