Applications of LC-MS in Toxicology covers all essential theoretical aspects of LC-MS.

Features:

• Includes technical details of the instrumentation, method development, optimisation and validation, quantification, maintenance and troubleshooting
• Covers in detail the application of LC-MS in areas such as: systematic toxicological analysis, drugs of abuse, therapeutic drug monitoring, doping in sport, pesticides, peptides and further potential forensic applications

Contents

Ionisation, Ion Separation and Ion Detection in LC-MS

• Ionisation
• Ion separation
• Ion detection
• Tandem mass spectrometry
• Acquiring and processing LC-MS data

Method Development and Optimisation of LC-MS

• Mass spectrometry
• Chromatography
• Sample preparation
• Preliminary method validation

Quantification Using LC-MS

• Samples and matrices
• Chromatography
• Ionisation
• Fragmentation and mass analysis
• Determining the concentration

Method Validation Using LC-MS

• Guidelines and literature on validation
• Validation parameters
• Experimental design for method validation

Identification and Confirmation Criteria for LC-MS

• Identification
• Confirmation of identity
• Value and role of mass spectral library searching in the identification of organic compounds
• Recommendations of regulatory bodies
• Specific difficulties in constructing an in-house LC-MS system
• Nature of databases for identification in LC-MS
• Requirement for pure standards
• Decision limits
• final considerations

Systematic Toxicological Analysis with LC-MS

• Molecular identification using LC-MS
• Chromatographic separation
• Sample preparation
• Practicality and efficiency of comprehensive LC-MS(-MS) ststematic toxicology analysis procedures

Analysis of Therapeutic Drugs with LC-MS

• Sample preparation for LC-MS(-MS) analysis of therapeutic drugs
• Separation systems for LC-MS(-MS) analysis of therapeutic drugs
• Ionisation and detection of therapeutic drugs in LC-MS(-MS) analysis
• Validation of LC-MS(-MS) procedures for the analysis of therapeutic drugs

LCMS of Drugs of Abuse and Related Compounds

• Gerneral methodological considerations
• Illicit drugs
• Therapeutic drugs with abuse potential
• LC-MS screening for drugs of abuse

LCMS in Doping Control

• Small molecules
• Large molecules

Pesticide Analysis Using LC-MS

• Sample matrices and analytes investigated
• Sample preparation
• LC-MS
• Quantification and confirmation of analytes
• Selected applications

Peptide Analysis Using LC-MS

• Analysis of peptides
• Analysis of proteins

LC-MS in Forensic Chemistry

• Lacrimators
• Inks
• Dyes in textile fibres
• Chemical warfare agents

Index

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