Calcium Signaling focuses on those processes that generate calcium signals to compile the first comprehensive exploration of calcium signaling research from a methodological standpoint.

Features:

• Explores the molecular basis, as well as the complex aspects, of calcium signaling
• Employs an easy-to-follow format in describing numerous research techniques, along with the pros and cons of each approach
• Discusses the major intracellular and surface membrane calcium channel molecules and intracellular effectors in terms of their molecular nature and biophysical behavior
• Introduces the application of high-throughput technology, and the use of genetically tractable organisms such as C. elegans, and yeast

Contents

• Fluorescent Calcium Indicators Based on BAPTA
• Fluorescent Indicators-Facts and Artifacts
• Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling: The Highs and Lows for Calcium Researchers
• Genetically Encoded Fluorescent Calcium Indicator Proteins
• Targeted Aequorins
• Electrophysiological Recordings of Ca2o Currents
• Molecular Biology of Ca2o Channels: Lessons from the TRP Superfamily
• Genetic and Molecular Characterization of Ca2o and IP3 Signaling in the Nematode Caenorhabditis elegans
• Calcium Signaling Networks in Yeast
• Nuclear Patch Clamp Electrophysiology of Inositol Trisphosphate Receptor Ca2o Release Channels
• Ryanodine Receptors
• Methods in Cyclic ADP-Ribose and NAADP Research
• Methods for Studying Calcium Pumps
• Measuring Single Cell and Subcellular Ca2o Signals
• Subcellular Compartmentalization of Calcium Signaling
• Apoptosis
• Calcium Entry Channels and Drug Discovery

Index

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