Cell Culture Technology for Pharmaceutical and Cell Based Therapies discusses aspects of cell biology and biochemical engineering used in pharmaceutical process development and manufacturing.

Features:

• Describes manipulations and the genetic engineering of cells for the production of biotechnology products
• Outlines novel techniques for the development of serum-free and chemically defined media formulations
• Addresses glycosylation and post translation of proteins and their importance for product quality
• Presents guidelines for upstream and downstream process development for the production of cell culture based pharmaceuticals
• Offers detailed guidance on the operation of bioreactors and production facility design
• Presents a comprehensive outline for drug formulation
• Describes the design of aeration, mixing, and process control for batch and perfusion bioreactors
• Details the optimization of protein expression from transient, batch, and perfusion systems
• Offers discussions on the use of mammalian cells, insect cells, and stem cells
• Discusses the emerging field of tissue engineering and its applications in the development of cell based therapies

Contents

• Cell Culture Technology: An Overview
• Recombinant DNA Technology and Cell Line Development
• Medium Development
• Cell Metabolism
• Protein Glycosylation
• Cell Culture Bioreactors
• Aeration, Mixing and Hydrodynamics in Bioreactors
• Instrumentation and Process Control
• Cell Culture Kinetics and Modeling
• Fed-Batch Cultivation Of Mammalian Cells For Production Of Recombinant Proteins
• Optimization of High Density Perfusion Bioreactors
• Cell Separation And Product Capture
• Downstream Processing
• Formulation, Finishing, and Packaging
• Validation Of Cell Culture Based Processes & Qualification Of Associated Equipment And Facility
• Facility Design
• Transient Expression of Proteins
• Large-Scale Propagation of Insect Cells
• Advances In Adult Stem Cell Culture
• Ex Vivo Culture Of Hematopoietic And Mesenchymal Stem Cells For Tissue Engineering And Cell-Based Therapies

Index

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