With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, Cell-Free Protein Synthesis covers the latest advances in the field.

The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Contents

1. Cell-free protein synthesis systems: historical landmarks, classification, and general methods
2. The constructive approach for cell-free translation
3. Functional genomic analysis using sequential cell-free protein synthesis
4. Cell-free technology for rapid production of patient-specific fusion protein vaccines
5. Bacterial cell-free system for highly efficient protein synthesis
6. The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics
7. The wheat germ cell-free protein synthesis system
8. Cell-free expression of integral membrane proteins for structural studies
9. Cell-free production of membrane protein in presence of detergents
10. Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering
11. Gene Cloning and Expression in Molecular Colonies
12. Large-scale batch reactions for cell-free protein synthesis

Index

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