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Culture of Animal Cells
A Manual of Basic Technique
Fifth Edition
by R. Ian Freshney

Culture of Animal Cells provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells.

Culture of Animal Cells contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Culture of Animal Cells, Fifth Edition includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.

Contents:

  1. Introduction
  2. Biology of the Cultured Cell
  3. Design and Layout of the Laboratory
  4. Equipping the Laboratory
  5. Aseptic Technique
  6. Laboratory Safety and Biohazards
  7. The Culture Environment: Substrate, Gas Phase, Medium and Temperature
  8. Preparation and Sterilization
  9. Disaggregation of the Tissue and Primary Culture
  10. Maintenance of the Culture: Cell Lines
  11. Cloning and Selection of Specific Cell Types
  12. Physical Methods of Cell Separation
  13. Characterization
  14. Induction of Differentiation
  15. The Transformed Phenotype
  16. Contamination
  17. Instability, Variation, and Preservation
  18. Quantitation and Experimental Design
  19. Measurement of Viability and Cytotoxicity
  20. Culture of Specific Cell Types
  21. Culture of Tumor Tissue
  22. Three-Dimensional Culture Systems
  23. Specialized Techniques
Index

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Culture of Animal Cells
A Manual of Basic Technique
Fifth Edition
by R. Ian Freshney

2005 642 pages $99.95 + shipping
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