Culture of Cells for Tissue Engineering is a step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering.

Features:

• Easy-to-use format with a two-part organization
• Logically organized—part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues
• Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
• End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book
• A section of elegant color plates to back up the figures in the chapte

Contents

Cell Culture

1. Basic principles of cell culture
2. Mesenchymal stem cells for tissue engineering
3. Human embryonic stem cell culture for tissue engineering
4. Cell sources for cartilage tissue engineering
5. Lipid-mediated gene transfer for cartilage tissue engineering

Tissue Engineering

6. Tissue engineering: basic considerations
7. Tissue engineering of articular cartilage
8. Ligament tissue engineering
9. Cellular photoencapsulation in hydrogels
10. Tissue engineering human skeletal muscle for clinical applications
11. Engineeered heart tissue
12. Tissue-engineered blood vessels
13. Tissue engineering of bone
14. Culture of neuroendocrine and neuronal cells for tissue engineering
15. Tissue engineering of the liver

Index

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