The Fusarium Laboratory Manual provides an overview of the biology of Fusarium and the techniques involved in the isolation, identification and characterization of individual species and the populations in which they occur.

Contents

Media – Recipes and Preparation

1. Media for Growing and Identifying Fusarium
2. Supplementary Identification Media
3. Media for Isolating Fusarium
4. Media for the Preparation of Natural Inocula
5. Synthetic and Semi-synthetic Media
6. Media for Sexual Crosses
7. Sterilization of Media and Materials

Techniques for Recovering Fusarium

1. Collecting strategy(ies)
2. Isolation Techniques – Plants
3. Isolation Techniques – Soil
4. Isolation Techniques – Spore Trapping and Air Sampling
5. Seed Disinfestation

Techniques for Growing and Maintaining Fusarium

1. Vegetative Propagation
2. Preparing Cultures for Identification
3. Single Spore Subcultures
4. Mutagenesis
5. Culture Preservation

Vegetative Compatibility Groups (VCGs)

1. History of and Genetic Basis Underlying Vegetative Compatibility 5.2 Overall Strategy for Determining if Strains are Vegetatively Compatible
2. Recovering and Identifying nit Mutants
3. Typical Pairing Protocols
4. Common Trouble Spots – HSI, crn, and NitMs
5. Characterizing a Population with VCGs

Fertility Concepts

1. Heterothallic, Homothallic and Pseudohomothallic
2. Mating Type
3. Population Effects of Mating Type
4. Male, Female, and Hermaphrodite
5. Crossing Protocols
6. Developing Female-Fertile Tester Strains
7. Species Identification Through Sexual Crosses

Nucleic Acid Analyses

1. DNA Extraction and Purification
2. PCR – Mating-Type Alleles
3. Amplified Fragment Length Polymorphisms (AFLPs)
4. Sequence Analysis and Sequenced Loci
5. Genetic Maps

A Brief History of Fusarium Taxonomy

Species Concepts in Fusarium

1. Generic Problems in Speciation in Fusarium
2. Morphological Species Concepts
3. Biological Species Concepts
4. Phylogenetic Species Concepts
5. How Many Strains Make a Species?
6. Species Names
7. Subspecific Terminology
8. A Species Concept for Fusarium

Teleomorphs of Fusarium

1. Taxonomy of Teleomorphs
2. General Teleomorph Characters
3. Sexual Development and Differentiation
4. Spore Killer
5. Anamorph-Teleomorph Connections

Practical Approaches to Identification

1. Overall Identification Strategy
2. The Diseased Plant and Its Geographic Origin
3. Native and Agricultural Populations
4. Culture Preparation
5. The Essence of Morphological Identifications
6. Beyond Morphology – Sexual Cross Fertility
7. Beyond Morphology – Molecular Diagnostics
8. The Special Case of Fusarium oxysporum
9. Differences Between Temperate and Tropical Regions

Morphological Characters

1. Macroconidia
2. Microconidia
3. Chlamydospores
4. Other Characters
5. Secondary Characters

Species Descriptions

Index

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ORDER NOW The Fusarium Laboratory Manual by John F. Leslie 2006 • 388 pages • $128.95 + shipping Texas residents please add 6.75 % sales tax