edited by Susan R. George
and Brian F. O'Dowd
G Protein-Coupled Receptor-Protein Interactions elucidates how GPCRs organize signal transduction and control intracellular activities.
G Protein-Coupled Receptor-Protein Interactions offers state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.
Contents:
- Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies
- Cooperativity in Ligand Binding to Deduce Receptor/Receptor Interaction
- Analysis of Receptor-Receptor Interaction Using Time-Resolved Fluorescence Resonance Energy Transfer
- Real Time Imaging of GPCR-medicated Arresting Translocation as a Strategy to Evaluate Receptor-Protein Interactions
- Localization of G Protein Coupled Receptors and Interacting Proteins in Membrane Microdomains
- Identification of Peptide Ligand Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling
- Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled Receptor-G Protein Complexes
- Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Cross-Linking Strategies to Analyze Receptor-Receptor Interactions
- Co-Immunoprecipitation as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions
- Use of MALDI-TOF and Mass Spectrometry in the Evaluation of Receptor-Protein Interactions
- Use of the Yeast Two-Hybrid System to Detect Receptor-Protein Interactions
- GST Fusion Protein and Pull-down Strategies to Evaluate Receptor-Receptor or Receptor-Protein Interactions
- G-Protein-Coupled Fusion to Monitor Integral Membrane Protein-Protein Interactions
- Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G-Protein-Coupled Receptors
- Electrophysiological Characterization of GPCR and Ligand-Gated Channel Crosstalk
Index
to see books • videos • cd-roms of related interest