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Pharmaceutical Book from C.H.I.P.S.

Green Fluorescent Protein
Properties, Applications, and Protocols
Second Edition
edited by Martin Chalfie

Green Fluorescent Protein provides real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Features:

  • Places emphasis on the rise of NFPs, NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors
  • Provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls

Contents

  • Discovery of Green Fluorescent Protein
  • Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems
  • Biochemical and Physical Properties opf Green Fluorescent Protein
  • The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design
  • Molecular Biology and Mutation of Green Fluorescent Protein
  • Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa
  • Evolution of Function and Color in GPF-Like Proteins
  • The Uses of Green Fluorescent Protein in Prokaryotes
  • The uses of Green Fluorescent Protein in Yeasts
  • Uses of GFP in Caenorhabditis Elegans
  • Green Fluorescent Protein Applications in Drosophila
  • The Uses of Green Fluorescent Protein in Plants
  • Uses of GFP in Transgenic Vertebrates
  • The Uses of Green Fluorescent Protein in Mammalian Cells
  • Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry
  • Pharmaceutical Applications of GFP and RCFP
  • Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns
  • Methods and Protocols

Index

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Green Fluorescent Protein
Properties, Applications, and Protocols
Second Edition
edited by Martin Chalfie

2005 464 pages $104.00 + shipping
Texas residents please add 6.75 % sales tax

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