Green Fluorescent Protein provides real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Features:

• Places emphasis on the rise of NFPs, NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors
• Provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls

Contents

• Discovery of Green Fluorescent Protein
• Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems
• Biochemical and Physical Properties opf Green Fluorescent Protein
• The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design
• Molecular Biology and Mutation of Green Fluorescent Protein
• Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa
• Evolution of Function and Color in GPF-Like Proteins
• The Uses of Green Fluorescent Protein in Prokaryotes
• The uses of Green Fluorescent Protein in Yeasts
• Uses of GFP in Caenorhabditis Elegans
• Green Fluorescent Protein Applications in Drosophila
• The Uses of Green Fluorescent Protein in Plants
• Uses of GFP in Transgenic Vertebrates
• The Uses of Green Fluorescent Protein in Mammalian Cells
• Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry
• Pharmaceutical Applications of GFP and RCFP
• Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns
• Methods and Protocols

Index

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