by Dalbir Singh
and S. B Mathur

Histopathology of Seed-Borne Infections is the first book to provide comprehensive coverage of seed infection and disease. It includes an up-to-date account on the development and structure of seed, pointing out the structural variations in seeds of the plant families to which most crop plants belong.

Histopathology of Seed-Borne Infections presents techniques and advice for histopathological studies, as well as a discussion on physiogenic seed disorders and the penetration of ovule/seed by fungi.

Features:

• Includes separate chapters on seed-borne microorganisms (fungi, bacteria, viruses, nematodes) and their diseases
• Covers the penetration of ovule/seed by fungi
• Discusses physiogenic seed disorders
• Provides common techniques and tips for histopathological studies

Contents

Introduction

• The Seed
• Microorganisms in Seed
• Histopathology

Reproductive Structures and Seed Formation

• Flower
• Sterile Appendages
• Fertile Appendages
• Nectaries
• Ovule
• Development and Structure of Female Gametophyte
• Fertilization
• Seed Development
• Seed Coat Development in Selected Genera

Structure of Seeds

• Constitution of Seeds
• External Morphology
• Internal Morphology
• Seed Structure in Selected Families

Penetration and Establishment of Fungi in Seed

• Environments of Ovule and Seed
• Nature of Pathogen
• Seed Infection in Developing Seeds
• Avenues of Infection in Threshed Seeds
• Mechanism of Penetration of Ovary/Fruit and Ovule/Seed Surfaces

Location of Fungal Hyphae in Seeds

• Severity of Infection and Location
• Primary Sites of Colonization
• Host-pathogen Interactions
• Mixed Infections
• Colonization of Seed Tissues
• Endophytes
• Implication of Internal Infection

Seed Infection of Bacteria

• Penetration
• Histopathology of Infected Seeds
• Survival in Seed

Seed Infection by Viruses

• Infection and Multiplication
• Cellular Contacts, Isolations and Transport Systems in Ovule and Seed
• Virus Movement
• Location in Reproductive Shoot, Ovule and Seed
• Cytopathological Effects
• Inactivation and Longevity of Viruses in Seed During Maturation and Storage

Seed Infection by Nematodes

• Penetration by Nematodes
• Histopathology
• Association of Nematodes and Bacteria
• Survival in Seed

Physiogenic or Non-Pathogenic Seed Disorders

• Mineral Nutrient Deficiency
• Humidity Effects

Microtechniques in Seed Histopathology

• Choice of Material
• Determination of Identity of Internal Mycelium Seed Softening
• Histological Methods
• Procedures for Preparing Some Reagents and Stains

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