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HPLC Made to Measure
A Practical Handbook for Optimization
edited by Stavros Kromidas

HPLC Made to Measure provides well-founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP-HPLC, NP-HPLC, micro- and nano-HPLC, and hyphenated techniques.


  • Presents applications using common optimization software, such as DryLab or ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, while their self-contained structure allows for targeted references
  • Offers concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors
  • Includes real-life reports from experienced users coming from the different areas of application, in particular the life sciences, such as proteomics or drug development.


Essential Aspects of Optimization

  • Principles of HPLC Optimization Illustrated by RP-Chromatography
  • Fast Gradient Separations
  • pH and Selectivity in Reversed-Phase Chromatography
  • Selecting the Correct pH-Value for HPLC
  • Optimization of Data Evaluation in Chromatography
  • Calibration Characteristics and Uncertainty indicating Starting-Points for Method Optimization

Optimization in Different LC-MODI

  • Comparison and Choice of Columns for RP-HPLC
  • Column Selectivity in Reversed-Phase Chromatography
  • The Use of Principal Component Analysis for the Characterisation of Reversed-Phase Liquid Chromatographic Stationary Phases
  • Chemometrics - A Powerful Tool for Handling a Large Number of Data
  • Linear Free Energy Relations -
  • Tools for Column Characterization and Method Optimization in HPLC?
  • Column Selectivity in Reversed-Phase Liquid Chromatography
  • Understanding Selectivity by Use of Suspended-State High Resolution Magic Angle Spinning NMR Spectroscopy
  • Optimization in Normal-Phase HPLC
  • Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection
  • Gel Filtration - Size Exclusion Chromatography of biopolymers
  • Optimization in Affinity Chromatography
  • Optimization of Chiral Separations in HPLC
  • µLC/nano LC - Optimization and Troubleshooting
  • Microchip-Based Liquid Chromatography -
  • Techniques and Possibilities
  • Ultra-Performance Liquid Chromatography

Hyphenated Techniques

  • Immunochromatographic Techniques
  • Enhanced Characterization and Comprehensive Analyses by 2-dimensional Chromatography
  • LC/MS - Hints and Recommendations for Optimization and Troubleshooting
  • LC-NMR-Coupling

Computer-Aided Optimization

  • Computer-Facilitated HPLC Method Development Using DryLab Software
  • ChromSword Software for Automated and Computer-Assisted Development of HPLC Methods
  • Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC

User Reports

  • NanoLC-MS/MS in Proteomics
  • Ways for the Verification of the Robustness in RP-HPLC
  • Separation of Complex Sample Mixtures
  • Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC-UV/MS/CLND


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HPLC Made to Measure
A Practical Handbook for Optimization
edited by Stavros Kromidas

2006 • 786 pages • $174.00 + shipping
Texas residents please add 6.75 % sales tax

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