Human Embryonic Stem Cells contains the first centralised collection of methods used in human embryonic stem cell biology.

Human Embryonic Stem Cells contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells.

Human Embryonic Stem Cells:

• Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells
• Centred solely on practical protocols for human (not mouse) embryonic stem cell research
• Includes extensive troubleshooting sections
• Addresses the different proclivities and behaviours of individual human embryonic cell lines
• Contains techniques currently known only to a small number of specialised laboratories worldwide

Contents

Obtaining and Culturing Human Embryonic Stem Cells

1. Organization and Good Aseptic Technique in the Human Embryonic Stem Cell Laboratory
2. Sourcing Human Embryonic Stem Cell Lines
3. Human embryo culture for human embryonic stem cell derivation
4. Derivation of Human Embryonic Stem Cell Lines
5. Standard Culture of Human Embryonic Stem Cells
6. Culture of Human Embryonic Stem Cells in Chemically Defined Conditions

Characterization of Undifferentiated Embryonic Stem Cells

7. Phenotypic Analysis of Human Embryonic Stem Cells
8. Genetic and Epigenetic Analysis of Human Embryonic Stem Cells

Manipulation of Human Embryonic Stem Cells

9. In Vivo Differentiation of Human Embryonic Stem Cells
10. In Vitro Differentiation of Human Embryonic Stem Cells
11. Differentiation of Human Embryonic Stem Cells into Extraembryonic Cell Types
12. Directed Differentiation of Human Embryonic Stem Cells Into:
    • Early Endoderm Cells
    • Hepatic Cells
    • Pancreatic Cells

13. Directed Differentiation of Human Embryonic Stem Cells Into:
    • Cardiomyocytes
    • Endothelial Cells
    • Osteogenic Cells
    • Hematopoietic in Vivo Repopulating Cells
    • Lymphocytes
    • Myeloid Cells

14. Directed Differentiation of Human Embryonic Stem Cells Into:
    • Forebrain Neurons
    • Dopaminergic Neuron
    • Spinal Motor Neurons

15. • Gene Targeting: Knock Out and Knock in by Homologous Recombination
    • RNA Interference in Human Embryonic Stem Cells
    • Generation of Gene Reporters Using Bacterial Artificial Chromosome Recombineering

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