Introduction to Plant Biotechnology covers all the important aspects of plant tissue culture.

Features:

• Includes gene cloning, isolation of plant genes, transposons, and gene tagging
• Details enzymes, vectors and cDNA and genome cloning and analysis of cloned DNA sequences
• Discusses crop improvement and an overview of the impact of biotechnology on crop improvement
• Gives application techniques and discusses its impact on crop biotechnology

Contents

Plant Tissue Culture

• Laboratory Organization
• Nutrition Medium
• Sterilization Techniques
• Types of Culture
• Micropropagation
• Cell Suspension and Secondary Metabolites
• In vitro Production of Haploids
• Protoplast Isolation and Fusion
• Somaclonal Variation
• Germplasm Storage and Cryopreservation

Genetic Material and its Organization

• Genetic Material
• Organization of DNA and Gene Expression

Recombinant DNA Technology

• Basic Techinques.
• Gene Cloning-Cuttting and Joining DNA Molecules.
• Gene-cloning Vectors
• Gene Cloning- cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences.
• Polymerase Chain Reaction
• In Vitro Mutagenesis
• Transposon Genetic Elements and Gene Tagging
• Gene Isolation
• Molecular Markers and Marker Assisted Selection
• Gene Transfer Methods
• Transgenics in Crop Improvement
• Genomics, DNA Chips, and Microarrays
• Intellectual Property Rights

Index

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