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Introduction to Plant Biotechnology
Second Edition
by H.S. Chawla

Introduction to Plant Biotechnology covers all the important aspects of plant tissue culture.

Features:

  • Includes gene cloning, isolation of plant genes, transposons, and gene tagging
  • Details enzymes, vectors and cDNA and genome cloning and analysis of cloned DNA sequences
  • Discusses crop improvement and an overview of the impact of biotechnology on crop improvement
  • Gives application techniques and discusses its impact on crop biotechnology

Contents

Plant Tissue Culture

  • Laboratory Organization
  • Nutrition Medium
  • Sterilization Techniques
  • Types of Culture
  • Micropropagation
  • Cell Suspension and Secondary Metabolites
  • In vitro Production of Haploids
  • Protoplast Isolation and Fusion
  • Somaclonal Variation
  • Germplasm Storage and Cryopreservation

Genetic Material and its Organization

  • Genetic Material
  • Organization of DNA and Gene Expression

Recombinant DNA Technology

  • Basic Techinques.
  • Gene Cloning-Cuttting and Joining DNA Molecules.
  • Gene-cloning Vectors
  • Gene Cloning- cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences.
  • Polymerase Chain Reaction
  • In Vitro Mutagenesis
  • Transposon Genetic Elements and Gene Tagging
  • Gene Isolation
  • Molecular Markers and Marker Assisted Selection
  • Gene Transfer Methods
  • Transgenics in Crop Improvement
  • Genomics, DNA Chips, and Microarrays
  • Intellectual Property Rights

Index

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Introduction to Plant Biotechnology
Second Edition
by H.S. Chawla

562 pages $48.00 + shipping
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