Chromatography Book from C.H.I.P.S.

Introduction to Modern Liquid Chromatography
Third Edition
edited by Sharon Andrew

Introduction to Modern Liquid Chromatography addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.

The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:

  • The basis of HPLC separation and the general effects of different experimental conditions
  • Equipment and detection
  • The column—the "heart" of the HPLC system
  • Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
  • Computer simulation, qualitative and quantitative analysis, and method validation and quality control
  • The separation of large molecules, including both biological and synthetic polymers
  • Chiral separations, preparative separations, and sample preparation
  • Systematic development of HPLC separations—new to this edition
  • Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms

Basic Concepts and the Control of Separation

  • The Chromatographic Process
  • Retention
  • Peak Width and the Column Plate Number N
  • Resolution and Method Development
  • Sample Size Effects
  • Reservoirs and Solvent Filtration
  • Mobile-Phase Degassing
  • Tubing and Fittings
  • Pumping Systems
  • Autosamplers
  • Column Ovens
  • Data Systems
  • Extra-Column Effects
  • Maintenance
  • Detector Characteristics
  • Introduction to Individual Detectors
  • UV-Visible Detectors
  • Fluorescence Detectors
  • Electrochemical (Amperometric) Detectors
  • Radioactivity Detectors
  • Conductivity Detectors
  • Chemiluminescent Nitrogen Detector
  • Chiral Detectors
  • Refractive Index Detectors
  • Light-Scattering Detectors
  • Corona-Discharge Detector (CAD)
  • Mass-Spectral Detectors
  • Other Hyphenated Detectors
  • Sample Derivatization and Reaction Detectors
The Column
  • Column Supports
  • Stationary Phases
  • Column Selectivity
  • Column Hardware
  • Column-Packing Methods
  • Column Specifications
  • Column Handling
Reversed-Phase Chromatography for Neutral Samples
  • Retention
  • Selectivity
  • Method Development and Strategies for Optimizing Selectivity
  • Nonaqueous Reversed-Phase Chromatography (NARP)
  • Special Problems
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange Chromatography
  • Acid-Base Equilibria and Reversed-Phase Retention
  • Separation of Ionic Samples by Reversed-Phase Chromatography (RPC)
  • Ion-Pair Chromatography (IPC)
  • Ion-Exchange Chromatography (IEC)
Normal-Phase Chromatography
  • Retention
  • Selectivity
  • Method-Development Summary
  • Problems in the Use of NPC
  • Hydrophilic Interaction Chromatography (HILIC)
Gradient Elution
  • Experimental Conditions and Their Effects on Separation
  • Method Development
  • Large Molecule Separations
  • Other Separation Modes
Computer-Assisted Method Development
  • Computer-Simulation Software
  • Other Method-Development Software
  • Computer Simulation and Method Development
Qualitative and Quantitative Analysis
  • Signal Measurement
  • Qualitative Analysis
  • Quantitative Analysis
Method Validation
  • Terms and Definitions
  • System Suitability
  • Documentation
  • Validation for Different Pharmaceutical-Method Types
  • Bioanalytical Methods
  • Analytical Method Transfer (AMT)
  • Method Adjustment or Method Modification
  • Quality Control and Quality Assurance
Biochemical and Synthetic Polymer Separations
  • Molecular Structure and Conformation
  • Special Considerations for Biomolecule HPLC
  • Role of Protein Structure in Chromatographic Behavior
  • Separation of Peptides and Proteins
  • Separation of Nucleic Acids
  • Separation of Carbohydrates
  • Separation of Viruses
  • Size-Exclusion Chromatography (SEC)
  • Large-Scale Purification of Large Biomolecules
  • Synthetic Polymers
Enantiomer Separations
  • Indirect Method
  • Direct Method
  • Peak Dispersion and Tailing
  • Chiral Stationary Phases and Their Characteristics
  • Thermodynamic Considerations
Preparative Separations
  • Equipment for Prep-LC Separation
  • Isocratic Elution
  • Severly Overloaded Separation
  • Gradient Elution
  • Production-Scale Separation
Sample Preparation
  • Types of Samples
  • Preliminary Processing of Solid and Semi-Solid Samples
  • Sample Preparation for Liquid Samples
  • Liquid-Liquid Extraction
  • Solid-Phase Extraction (SPE)
  • Membrane Techniques in Sample Preparation
  • Sample Preparation Methods for Solid Samples
  • Column-Switching
  • Sampe Preparation for Biochromatography
  • Sampe Preparation for LC-MS
  • Derivatization in HPLC

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Introduction to Modern Liquid Chromatography
Third Edition
edited by Sharon Andrew

2010 • 912 pages • $124.00 + shipping
Texas residents please add 6.75 % sales tax

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