This reference/text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding.

Lipid Biotechnology offers an in-depth review of the most recent principles and approaches utilized in the development and design of lipids for cosmetic, industrial, pharmaceutical, and food products.

Discussing a variety of lipid-active enzymes from animal, plant, fungal, and microbial sources, it covers:

• modern tehcniques in genetic engineering for the modification of conventional crops
• biosynthetic pathways for cutin polymers, flavor volatiles, oxylipins, and terpenoid compounds
• use of lipases and phospholipases in the creation of structured lipids and fats, including cocoa butter, low-calorie fats, and Betapol
• emerging methods utilizing supercritical carbon dioxide as a benign solvent for lipid analysis, fractionation, and enzymatic reaction
• reaction conditions, reactor design, solvent selection, immobilization technology, and enzyme sources for optimal large-scale manufacturing
• formation of oxylipins through the lipoxygenase pathway, as well as other fatty acids
• and more!

Contents

1. Oilseed Quality Improvement
   1. Metabolic Engineering of Fatty Acid Biosynthesis
   2. Biochemistry and Biotechnology of Triacylglycerol Accumulation in Plants
   3. Redirecting Lipid Metabolism in Plants
   4. Perspectives on the Production of Industrial Oils in Genetically Engineered Oilseeds
   5. New Gene Combinations Governing Saturated and Unsaturated Fatty Acid Composition in Soybean
   6. Strategies for the Development of NuSun Sunflower Hybrids
   7. Biosynthesis of Ricinoleic Acid for Castor-Oil Production

2. Oxylipins: Formulation, Potential Uses and Their Role in Defense
   8. Enzymatic Transformation of Lipids as a Plant Defense Mechanism
   9. Biocatalysis by the Plant Lipoxygenase Pathway: Oxygenated Fatty Acid Production and Hydroperoxide Lyases
   10. Biocatalysis by the Plant Lipoxygenase Pathway: Hydroperoxide-Metabolizing Enzymes
   11. Prostaglandin-like Oxylipins in Plants: Biosynthesis, Molecular Signaling, and Response to Environment
   12. Cutin Monomers: Biosynthesis and Plant Defense
   13. Structural Diversity of Marine Oxylipins
   14. Formation of Oxygenated Fatty Acids by Fungal Enzymes
   15. Enzymic Formation of Flavor Volatiles from Lipids
   16. Terpenoid-Based Defense in Plants and Other Organisms

3. Lipases and Food Nutrition
   17. Lipases: Structure, Function, and Properties
   18. Industrial Uses of Lipase
   19. Plant Lipases as Biocatalysts
   20. Phospholipases in Enzyme Engineering of Phospholipids for Food, Cosmetics, and Medical Applications
   21. Enzymatic Synthesis of Structured Lipids
   22. Enzymatic Production of Betapol and Other Specialty Fats
   23. Enzyme-Mediated Modification of Milkfat
   24. Enzymatic Enrichment of Polyunsaturated Fatty Acids
   25. Production of Tailor-Made Polyunsaturated Phospholipids Through Bioconversions

4. Biocatalysts in Nonfood Applications
   26. Enzymatic Preparation of Chiral Pharmaceutical Intermediates by Lipases
   27. Production of C₂₀ Polyunsaturated Fatty Acids by an Arachidonic Acid-Producing Fungus Mortierella alpina 1S-4 and Related Strains
   28. Microbial Transformations of Steriods
   29. Enzymatic Approaches to the Production of Biodiesel Fuels
   30. Lipase-Catalyzed Synthesis of Value-Added Fatty Acid Esters
   31. Microbiological Conversions of Fatty Acids to Value-Added Products
   32. Biosynthesis and Applications of Glycolipid and Lipopeptide Biosurfactants
   33. Application of Lipolytic Enzymes in Detergents
   34. Supercritical Fluid Technology for Lipid Extraction, Fractionation, and Reactions
   35. Enzyme Reactions in Supercritical Fluids

Index

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