Basic Mass Spectrometry Handbook
from C.H.I.P.S.

Mass Spectrometry:
Principles and Applications
Third edition
by Edmond de Hoffmann

Mass Spectrometry, Third edition provides a complete overview of the principles, theories and key applications of modern mass spectrometry.

All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter.

Emphasis is placed throughout the text on optimal utilization conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra.

This new edition has been thoroughly revised and updated. It contains numerous examples, references and a series of exercises of increasing difficulty to encourage understanding.

Updates include:

  • Increased coverage of MALDI and ESI
  • more detailed description of time of flight spectrometers
  • new material on isotope ratio mass spectrometry
  • an expanded range of applications


Ion Sources

  • Electron Ionization
  • Chemical Ionization
  • Field Ionization
  • Fast Atom Bombardment and Liquid Secondary Ion Mass Spectrometry
  • Field Desorption
  • Plasma Desorption
  • Laser Desorption
  • Matrix-Assisted Laser Desorption Ionization
  • Thermospray
  • Atmospheric Pressure Ionization
  • Electrospray
  • Atmospheric Pressure Chemical Ionization
  • Atmospheric Pressure Photoionization (APPI)
  • Atmospheric Pressure Secondary Ion Mass Spectrometry (APSIMS)
  • Inorganic Ionization Sources
  • Gas-Phase Ion-Molecule Reactions
  • Formation and Fragmentation of Ions: Basic Rules

Mass Analyzers

  • Quadrupole Analyzers
  • Ion Trap Analyzers
  • The Electrostatic Trap or “Orbitrap”
  • Time-of-Flight Analyzers
  • Magnetic and Electromagnetic Analyzers
  • Ion Cyclotron Resonance and Fourier Transform Mass Spectrometry
  • Hybrid Instruments

Detectors and Computers

  • Detectors
  • Computers

Tandem Mass Spectrometry (MS/MS)

  • Tandem Mass Spectrometry in Space or in Time
  • Tandem Mass Spectrometry Scan Modes
  • Collision-activated or Collision-induced Dissociation (CAD or CID)
  • Other Methods of Ion Activation
  • Reactions Studied in MS/MS
  • Tandem Mass Spectrometry Applications

Mass Spectrometry/Chromatography Coupling

  • Elution Chromatography Coupling Techniques
  • Chromatography Data Acquisition Modes
  • Data Recording and Treatment

Analytical Information

  • Mass Spectrometry Spectral Collections
  • High Resolution
  • Isotopic Abundances
  • Low-mass Fragments and Lost Neutrals
  • Number of Rings or Unsaturations
  • Mass and Electron Parities, Closed-shell Ions and Open-shell Ions
  • Quantitative Data

Fragmentation Reactions

  • Electron Ionization and Fragmentation Rates
  • Quasi-equilibrium and RRKM Theory
  • Ionization and Appearance Energies
  • Fragmentation Reactions of Positive Ions
  • Fragmentation Reactions of Negative Ions
  • Charge Remote Fragmentation (CRF)
  • Spectrum Interpretation

Analysis of Biomolecules

  • Biomolecules and Mass Spectrometry
  • Proteins and Peptides
  • Oligonucleotides
  • Oligosaccharides
  • Lipids
  • Metabolomics



  • Nomenclature
  • Abbreviations
  • Fundamental Physical Constants
  • Table of Isotopes in Ascending Mass Order
  • Table of Isotopes in Alphabetical Order
  • Isotopic Abundances in % for Various Elemental Compositions CHON (M = 100%)
  • Gas-Phase Ion Thermochemical Data of Molecules
  • Gas-Phase Ion Thermochemical Data of Radicals
  • Literature on Mass Spectrometry
  • Mass Spectrometry on Internet


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Mass Spectrometry
Principles and Applications
Third edition
by Edmond de Hoffmann

2008 • 407 pages • $159.00 + shipping
Texas residents please add 6.75 % sales tax

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