edited by Sidney A. Simon and Miguel A. L. Nicolelis
Written by world experts in the field of neuronal chemoreception, Methods in Chemosensory Research describes new molecular, electrophysiological, genetic, behavioral, psychophysical, and imaging techniques that have recently been adapted to investigate the basic neuronal mechanisms underlying chemoreception: taste, olfaction and chemical-induced irritation. This unique book includes a wide variety of new and innovative imaging methods, electrophysiological methods, methods to investigate development, and various aspects of behavioral testing.
Covering the entire spectrum of chemoreception, each section not only describes what information can be obtained by using each method, but also shows how to apply each method to obtain reliable results. The book presents a comprehensive view of how the brain uses information about chemical stimuli that interact with receptors in the nose and tongue. Methods in Chemosensory Research gives you the new tools to learn how chemicals affect activity from the genetic to the behavioral levels.
Features:
- Combines new and innovative methods to investigate new and novel aspects of taste and olfaction
- Describes behavioral studies of the taste and olfactory systems
- Includes modern chemosensory research such as identifying new genes, imaging (calcium, voltage-dependent dyes, NMR), rapid kinetics, behavioral testing, recordings from awake behaving animals
- Describes single and multi-electrode recordings from awake behaving animals, recordings from isolated taste cells, and various brainstem nuclei and shows how these data can be interpreted
Contents
Behavioral Studies
- Psychophysical Measurement of Oral Chemesthesis
- Behavioral Methods in Olfactory Research with Rodents
Molecular and Cell Biology of Taste and Olfaction
- Recordings from Vertebrate Olfactory Receptor Neurons: From Isolated Cells to
Intact Epithelial Preparations
- Recordings from Olfactory Receptor Neurons in the Rat in Vivo
- Voltage-Sensitive and Calcium-Sensitive Dye Imaging of Activity in the Olfactory Bulb: Presynaptic Inhibition, Maps of Receptor Cell Input, and Oscillations
- Gustatory System Development: New Experimental Approaches in Amphibian and Mammalian Embryos
- Mammalian Taste Receptors
- Researching Isolated Taste Receptor Cells: Deciphering Transduction Cascades with Patch-Clamp and Calcium: Imaging Techniques
- Rapid Kinetic Measurements in Chemosensory Systems
- Electrophysiological Recordings of Mammalian Taste
Higher-Order Studies in Taste and Olfaction
- Activation of Neurons in the Trigeminal Subnucleus Caudalis (Vc) by Irritant Chemical Stimulation: Extracellular Single- Unit Recording and c-fos Immunohistochemical Methods
- Methodological Considerations for Electrophysiological Recording and Analysis of Taste-Responsive Neurons in the Brainstem of the Rat
- Electrophysiological Analysis of Olfactory Coding in the CNS
- Electrophysiological Studies of Gustation in Awake Rats
- Recording from Single Neurons in the Primary Taste Cortex of the Alert Macaque
- Olfactory Learning and the Neurophysiological Study of Rat Prefrontal Function
- Olfactory Event-Related Potentials
- Application of Functional MRI in Olfactory Studies
- Functional Imaging of Olfactory Activation in the Human Brain
Index
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