A practical, hands-on guide to using the microscope to analyze activated sludge in wastewater treatment!

Microscopic Examination of the Activated Sludge Process explores and explains:

• Microscopy, including microscopic measurements and techniques
• Directions for preparing and applying microbiological stains and immobilizing agents and techniques for preparing wet mounts and smears
• How to identify various types of organisms, including: floc particles and foam; protozoa; rotifers; worms and worm-like organisms; crustaceans; filamentous organisms; and algae and fungi
• The collection, evaluation, and presentation of observations

This straightforward guide includes figures, tables, worksheets, photomicrographs, and black-and-white drawings of many living, microscopic components of the activated sludge process. It equips plant operators and technicians to monitor, regulate, and troubleshoot the treatment processes and also serves as a valuable resource for research professionals and sanitary engineers in wastewater treatment.

Contents

Part I: Overview

1. Introduction
2. Mixed Liquor Biota Food Chain
3. Samples
4. Safety

Part II: Microscopy

5. Rationale for Microscopy
6. The Microscope
7. Microscopic Measurements
8. The Stereoscopic Binocular Microscope
9. Equipment and Supplies
10. Wet Mounts and Smears
11. Staining Techniques
12. Dispersed Growth

Part III: The Bulk Solution

13. Particulate Material
14. Spirochetes

Part IV: Floc Particles and Foam

15. Floc Particles
16. Tetrads
17. Zoogloeal Growth
18. Foam

Part V: Protozoa

19. Protozoa
20. Relative Predominance of Bacteria and Protozoa

Part VI: Rotifers

21. Rotifers

Part VII: Worms and Wormlike Organisms

22. Free-Living Nematodes
23. Gastrotriches
24. Water Bears
25. Bristleworms
26. Bloodworms
27. Sludge Worms

Part VIII: Crustaceans

28. Copepods and Cyclops
29. Water Fleas
30. Ostracoda

Part IX: Filamentous Organisms

31. Filamentous Organisms

Part X: Algae and Fungi

32. Algae
33. Fungi

Part XI: Collection, Evaluation, and Presentation of Observations

34. Microscopic Set-up and Rating Tables
35. Worksheets
36. Report of Microscopic Examination

Abbreviations and Acronyms
Chemical Compounds
Index

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