This self-contained book provides modern and practical analytical
methodologies for the assays in foods, pharmaceuticals and clinical samples.
It covers the fat-soluble Vitamins: A, beta-carotene, D, E and K as well as
the water soluble vitamins, C, folate, thiamin, biotin, pyridoxin and cyancobalims.
Contents
Part I: FAT-SOLUBLE VITAMINS
Chapter 1: Vitamin A
Sample Collection, Handling, and Storage
Sample Preparation
Reference Standards
High-performance Lliquid Chromatography
Other Liquid Chromatographic Methods
Gas Chromatography
Capillary Electrophoresis
Bioassays
Chapter 2: Vitamin D
Origins of Vitamin D2 and Vitamin D3
Metabolism and Biologic Functions of Vitamin D
Measurement of Vitamin D Bioactivity
Modern Methods for Determining the Vitamin D Content in Blood
Strategies for Determining Circulating Concentrations of 25-Hydroxyvitamin D
Strategies for Determining Circulating Concentrations of 1.25-Dihydroxyvitamin D
Measurement of 24,25-Dihydroxyvitamin D and 25,26-Dihydroxyvitamin D
Clinical Utility for Assays for Vitamin D and Its Metabolites
Chapter 3: Determination of Tocopherols and Tocotrienols in Foods and Tissues
Vitamin E Compounds in foods and Tissues
High-Performance Liquid Chromatography
Gas Chromatography
Supercritical Fluid Extraction and Chromatography
Determination of Stereoisomers
Future Development
Chapter 4: Vitamin K
Determination of Phylloquinone by HPLC
Determination of Phylloquinone Metabolites and Hydrogenation Products
Determination of Menaquinones by HPLC
Other Indicators of Vitamin K Nutritional Status
Chapter 5: Simultaneous Determination of Fat-Soluble Vitamins in food, Feed, and Serum
Simultaneous Analysis of Fat-Soluble Vitamins in Food and Feed
An Integrated Approach to Fat-Soluble Vitamin Analysis of Foods
Simultaneous Analysis of fat-Soluble Vitamins in Serum or Plasma
Part 2: WATER-SOLUBLE VITAMINS
Chapter 6: Thiamine
Properties
Methodology
Chapter 7: Riboflavin
Properties
Methodology
Chapter 8: Vitamin B6
Processing of Standards and Samples
Fluorometric Methods
Enzymatic Methods
Gas Chromatography/Mass Spectrometry
High-Performance Liquid Chromatography
Electrophoresis
Microbiological Assay
Immunoassay
4-Pyridoxic Acid
Other Metabolites
Assessment of Vitamin B6 Status
Chapter 9: Vitamin B12
Cobalimin Binding Proteins
Materials
Preparation of Binding Proteins
Standardization of Binding Proteins
Extraction of Cbl from Serum or Plasma
Extraction of Cbl from Cells or Tissues
Assay Protocol
General Comments on Assay Protocol
Interpretation of Results
Chapter 10: Niacin
General Methods
High-Performance Liquid Chromatography
Capillary Electrophoresis
Improvement of Chemical and Biological Methods
Electrochemical Detection
Chapter 11: Pantothenate
Biological Importance
Derivatives and Salts of Pantothenic Acid
Analytical Methods for Pantothenic Acid
Analytical Methods for Measuring CoA and Intermediates
Chapter 12: Biotin
Microbiological Assays
Avidin-Binding Assays
Microbial Versus Avidin-Binding Assays
Chromatographic Procedures
Radioisotopes of Biotin
Analysis of Biotin-Dependent Carboxylases
Biotin Metabolites in Biological Fluids
Biotin Metabolites in Food
Chapter 13: Vitamin C
Stabillity, Sensitivity, and Substance Interference
Colorimetric/Spectrometric Assays
Fluorometric Assays
Gas-Liquid Chromatography
Enzymatic Assays
Capillary Zone Electrophoresis
High-Performance Liquid Chromatography
Assays of Semidehydroascorbic Acid
Flow Injection Assay
In Vivo Assays
Assay Comparisons
Summary and Conclusions
Index
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