Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.
The methods and tools needed to distinguish target molecules from others by means of tailor-made receptors are constantly growing in importance and complexity.
Molecular Imprinting gives a concise and highly up-to-date overview of the remarkable progress made in this field in the last five years.
The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.
Contents:
- Introduction
- Importance of Receptor Molecules in Advanced Science and Technology
- Naturally Occurring Receptors
- Artificial Receptors
- Receptors for Practical Applications
- Why is the Molecular Imprinting Method so Promising?
- Fundamentals of Molecular Imprinting
- General Principles of Molecular Imprinting
- Covalent Imprinting and Non-covalent Imprinting
- Advantages and Disadvantages of Covalent and Non-covalent Imprinting
- History
- Experimental Methods (1) - Procedures of Molecular Imprinting
- Reagents and Experimental Procedures
- Covalent Imprinting
- Non-covalent Imprinting
- "Dummy Molecular Imprinting"
- Experimental Methods (2) - Evaluation of Imprinting Efficiency
- Chromatographic Experiments
- Batchwise Guest-Binding Experiments
- Determination of Guest-Binding Constants
- Spectroscopic Anatomy of Molecular Imprinting Reactions
- Structures of Adducts in the Pre-organization Step
- Determination of the Binding Constant K for the Formation of Monomer-Template Adduct
- Relationship between K Value and Imprinting Efficiency
- Structure of Guest-Binding Site
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- Flow Chart of a Typical Molecular Imprinting
- Choice of Agents
- Polymerization
- Packing of Imprinted Polymer into the HPLC Column
- Quantitative Evaluation of Imprinting Efficiency
- Applications of Molecularly Imprinted Polymers
- Sensor Applications
- Signaling Polymers
- Molecularly Imprinted Sorbent Assays
- Molecularly Imprinted Membranes
- Affinity-based Solid-phase Extraction
- In Situ Preparation of Imprinted Polymers
- Molecularly Imprinted Catalysts
- Recent Challenges and Progress
- Molecular Imprinting in Water
- Use of Two Kinds of Functional Monomers for Cooperative Recognition
- Inorganic Gel as the Matrix for Molecular Imprinting
- Artificial Enzyme (Molecular Catalyst) by Molecular Imprinting
- Conclusions and Prospects
Index
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