This reference discusses the impact that Molecular Biology and Genetic Engineering have had on the methods and strategies of modern pharmaceutical and medicinal chemistry, transforming virtually every step in the drug development process, from target finding to drug design and ADMET analysis.

Covers:

• Cellular Assays in Drug Discovery
• Transgenic Models
• Reporter Gene Assay Systems
• Stereoselective Synthesis with Recombinant Enzymes
• Nucleic Acid Drugs
• Enantioseparation of Chiral Drugs
• NMR-based Drug Discovery
• Pharmacogenomics and Toxicogenomics

With its broad coverage and up-to-date treatment, this volume is essential reading for all pharmaceutical chemists, molecular biologists and medical researchers using molecular biology tools to study drugs and their action.

Contents

1. Molecular Targets
   1. Cellular Assays in Drug Discovery
   2. Gene Knockout Models
   3. Reporter Gene Assay Systems for the Investigation of G-protein-coupled Receptors
   4. From the Human Genome to New Drugs: The Potential of Orphan G-protein-coupled Receptors

2. Synthesis
   5. Stereoselective Synthesis with the Help of Recombinant Enzymes
   6. Nucleic Acid Drugs

3. Analysis
   7. Recent Trends in Enantioseparation of Chiral Drugs
   8. Affinity Chromatography
   9. Nuclear Magnetic Resonance-based Drug Discovery
   10. 13C- and 15N-Isotopic Labeling of Proteins
   11. Application of Antibody Fragments as Crystallization Enhancers

4. Kinetics, Metabolism and Toxicology
   12. Pharmacogenetics: The Effect of Inherited Genetic Variation on Drug Disposition and Drug Response
   13. Pharmacogenomics of Bioavaliability and Elimination
   14. Toxicogenomics: Integration of New Molecular Biological Tools in Toxicology

Index

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