Protein Book from C.H.I.P.S.

Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics
edited by Mark Chance

Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins.

Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics includes footprinting and plistex specifically, setting this book apart from the competition.


1. Overview of Mass Spectrometry Technologies for Examining Protein Structure: Current and Future Directions

  • Hydrogen/Deuterium Exchange Mass Spectrometry
  • Hydroxyl-Radical-Mediated Protein Footprinting
  • Chemical Cross-linking
2. Hydrogen Exchange Mass Spectrometry: Principles and Capabilities

  • The Chemistry of Hydrogen Exchange
  • HX Mechanisms in Proteins
  • Deuterium Incorporation into Proteins
  • Measuring HX with Mass Spectrometry
  • Capabilities of HX MS in Structural Biology
3. Covalent Labeling Methods for Examining Protein Structure and Protein Interactions

  • Chemistry of Hydroxyl Radical Footprinting
  • Mass Spectrometry Approaches for Quantitative Protein Footprinting
  • Examples of Various Methods that Generate Hydroxyl Radicals in Solution to Examine Protein Structure
  • The Future: Hybrid Approaches that Combine Experimental and Computational Data
4. Complementary Methods for Structure Determination: Hydroxyl-Radical-Mediated Footprinting and Deuterium Exchange Mass Spectrometry as Applied to Serpin Structure

  • Technical Comparison of Hydroxyl-Radical-Mediated Footprinting and H/D Exchange Methodologies
  • Structural Mass Spectrometry Data
  • Solvent Accessibility
  • Dynamics
  • Significance for Serpin Structure and Function
5. Deuterium Exchange Approaches for Examining Protein Interactions: Case Studies of Complex Formation

  • Interactions of Regulatory and Catalytic Subunits of Protein Kinase A
  • Allostery in Protein-Protein Interactions Revealed by H/D Exchange
  • Interactions of the Inhibitor IkBα with the Transcription Factor NF-kB
6. Hydrogen/Deuterium Exchange Studies of Viruses

  • Overview of Virus Lifecycles
  • Structural Investigations of Viral Capsids
  • Dynamics of Viral Capsids
  • Hydrogen/Deuterium Exchange Studies of Virus Capsid Structure
  • Hydrogen/Deuterium Exchange Studies of Viral Protein Dyanmics
  • Technical Aspects of Performing Hydrogen/Deuterium Exchange Experiments on Viruses
7. Use of Enhanced Peptide Amide Hydrogen/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions

  • Theory of H/D Exchange
  • Overview of DXMS Technology for Protein-Protein Interactions
  • DXMS of Human Growth Hormone and Its Binding Protein
  • DXMS of PKA Regulatory Subunits
  • DXMS of PKA R-Submit D/D Domains and D-AKAP2 AKB Domain
  • Epitope Mapping by DXMS
8. Cross-linking as a Tool to Examine Protein Complexes: Examples of Cross-linking Strategies and Computational Modeling

  • Cross-linking Strategies
  • Cross-linking Methodology
  • challenges Associated with Combining Cross-linking with Mass Spectrometry
  • Advances in Mass Spectrometry Instrumentation and Capabilities
  • Novel Cross-linking Reagents for Mass Spectrometry
  • Analytical Software
  • Using Cross-linking Distance Constraints to Build Experimental Models of Protein Complexes
9. Complex Formation in the Actin Cytoskeleton: Cross-linking Tools to Define Actin Protein Structure and Interactions

  • Mapping Cross-linking with Methods Other than Mass Spectrometry
  • Actin-Actin Cross-linking
  • Intrastrand Cross-linked Actin between GLN41 and CYS374
  • Regulation of Cytoskeleton by ABPs and Mapping their Interfaces with Actin by Cross-linking
  • Cross-linking of Actin and Muscle Proteins—Examples of Experimental Approaches
  • Conclusing Remarks
10. Computational Approaches to Examining Protein-Protein Interactions: Combining Experimental and Computational Data in the Era of Structural Genomics

  • Interactome in Structural Genomics
  • Importance of Computational Methods in Structural Genomics
  • Combining Computational Method with Experimental Data in Modeling the Structure of Protein Binary Complex
11. Studies of Intact Proteins and Protein Complexes: ESI MS Approaches

  • Tertiary Structure Integrity and Conformational Heterogeneity (Charge State Distributions).
  • Quaternary Structure Integrity and Composition of Non-Covalent Complexes
  • Functional Competence
  • Flexibility Maps and Binding Interfaces
  • Gas Phase Ion Chemistry and Its Influence of the Measurement of Protein Properties in Solution
  • Challenges and Future Outlook
12. Two Approaches to Mass Spectrometric Protein Footprinting: PLIMSTEX and FPOP

  • Introduction: Protein-Ligand Interactions by Mass Spectrometry, Titration, and Hydrogen/Deuterium Amide Exchange and Fast Photochemical Oxidation of Proteins
  • Protein-Ligand Interactions by Mass Spectrometry, Titration, and H/D Amide Exchange (PLIMSTEX)
  • Applications of PLMSTEX
  • Self-Association of Insulin: A Protein/Protein Interaction
  • Features of PLMSTEX
  • Fast Photochemical Oxidation of Proteins: An Example of Fast Protein Footprinting
  • Features of FPOP


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Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics
edited by Mark Chance
2008 • 272 pages • $88.95 + shipping
Texas residents please add 6.75 % sales tax

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