Plant Pathogen Detection and Disease Diagnosis
Second Edition
Revised and Expanded
by P. Narayanasamy

This totally revised and expanded reference provides the most recent information on the detection, identification, and differentiation of all microbial plant pathogens-presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical, and molecular properties.

This book also contains current methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.

It also discusses novel enhancements in diagnostic sensitivity, reliability, and rapidity and covers:

• Development of effective crop disease management systems using diagnostic methods
• Up-to-the-minute techniques to prevent fungal, bacterial, phytoplasmal, viral, and viroid pathogens
• Newly "imported" diseases affecting crop economics
• State-of-the-art procedures for the protection of numerous agroecological environments
• And more!

This is an informative source for plant pathologists, physiologists, virologists, and biochemists; horticulturists; botanists; microbiologists; environmental scientists; agronomists; agriculturists; foresters; and upper-level undergraduate and graduate students in these disciplines.

Contents

1. Introduction
   • Nature and Causes of Plant Diseases
   • Disease Diagnosis and Management Strategies

2. Characteristics of Pathogenic Microbes
   • Light Microscopy
   • Methods of Isolation of Pathogens
   • Fungi
   • Bacteria
   • Phytoplasmas
   • Viruses
   • Viroids

3. Symptoms of Plant Diseases
   • External Symptoms
   • Cytopathological Characteristics
   • Molecular Genetics of Pathogenicity

4. Dissemination of Plant Pathogens
   • Transmission of Plant Viruses
   • Transmission of Viroids
   • Transmission of Bacterial Pathogens
   • Transmission of Fungal Pathogens

5. Cross Protection

6. Chemodiagnostic Methods
   • Detection of Plant Viruses
   • Detection of Phytoplasmas
   • Detection of Fungal Pathogens
   • Detection of Bacterial Pathogens
   • Detection of Viroids

7. Electron Microscopy
   • Preparation of Support Films
   • Calibration of the Electron Microscope
   • Techniques for Identification of Plant Viruses
   • Techniques for Detection of Phytoplasmas
   • Techniques for Detection of Fungal Pathogens
   • Techniques for Detection of Bacterial Pathogens
   • Scanning Electron Microscopy

8. Seriodiagnostic Methods
   • Detection of Plant Viruses
   • Detection of Phytoplasmas
   • Detection of Plant Pathogenic Bacteria
   • Detection of Fungal Pathogens
   • Detection of Viroids

9. Nucleic Acid-Based Techniques
   • Hybridization Methods
   • Detection of Plant Viruses
   • Detection of Viroids
   • Polymerase Chain Reaction
   • Detection of Phytoplasmas
   • Detection of Fungal Pathogens
   • Detection of Bacterial Pathogens

10. Detection of Double-Stranded RNAs

11. Diagnosis and Monitoring of Plant Diseases
    • Plant Disease Diagnostic Centers
    • Plant Quarantines

Index

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