Producing Biomolecular Substances with Fermentors, Bioreactors, and Biomolecular Synthesizers examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins.

Features:

• Presents practical techniques, time-proven methods, and essential applications
• Includes the declassified text of the CIA and Defense Intelligence Agency Report of 28 May, 2003: Iraqi Mobile Biological Warfare Agent Production Plants
• Delineates what must be done and how to do it effectively, followed by the formula for reaching the necessary value
• Contains a biotech glossary, numerous references, charts, illustrations, and an extensive index

Contents

Introduction

• Biotechnology
• Bioproduction
• The Bioprocess
• Cell Lines
• Cell-Culture Process
• Separation, Recovery, Purification
• Virus and Foreign-DNA Removal
• Quality Assurance

Technology Overview

• Biomolecular Foundations
• Cellular Variation
• Key Molecular Interactions
• Base-Pair Complementarity
• Genetic Coding
• Interrupting Gene Segments
• Sequence Determinations
• Cloning
• Synthesized DNA
• Cell Lines
• Expression Systems

Introduction to the Bioprocess

• Optimizing Product Yield
• Kinetic Models
• cGMP Standards
• Contract Manufacturing
• Complex Mixtures
• Biomolecular Products
• Joint Manufacturing
• Downscaling to Scale Up
• Large-Scale Bioprocesses
• Scaling-Up In-House or in a Contract Facility
• Lab-to-Pilot-Plant-to-Production
• Increasing Bioprocess Scale
• Differing Features at Larger Scale
• Simulating Environment at Scale
• Detailed Specifications
• Impact on Downstream Bioprocessing
• FDA Biologics Review Process
• Regulating Biological Products

Recombinant DNA Materials and Methods

• Cloning
• Screening and Selection
• DNA Synthesis
• Cell Lines
• Expression Systems
• Vector Construction
• Prokaryotic and Eukaryotic Growth Characteristics
• Expression Levels
• Intra- vs. Extra-cellular Expression
• Intracellular Expression
• Extracellular Expression
• Glycosylation
• Mammalian Cell Glycosylation
• Insect Cell Glycosylation
• General Considerations
• Further Post-translational Modifications
• Economic Concerns
• Regulatory Concerns
• Manufacturers' Directory

Enzymes

• Enzyme Protein Engineering
• Transformation in Non-Aqueous Systems
• Restriction Enzymes
• Restriction-Enzyme Classes
• Class I Restriction Enzymes
• Class II Restriction Enzymes
• Class III Restriction Enzymes
• Restriction-Enzyme Specificity
• Star Activity
• Enzyme Families and Compatible Ends
• Isoschizomers
• Reverse Transcriptases
• DNA and RNA Nucleases
• Manufacturers' Directory

DNA-Amplification Reagents

• Amplification by DNA Synthesis (PCR)
• Amplification by RNA Transcription (TAS and 3SR
• Amplification by Ligation (LAR, LCR, LAS)
• Amplification by RNA Replication (Q Replicase)
• Sequence-Based Amplification
• Manufacturers Directory

Cell-Culture Media

• Prokaryotic Cell-Culture Medium
• Eukaryotic Cell-Culture Medium
• Defined Medium
• Serum
• Other Sera
• Serum Contamination
• Serum-Free (Defined) Medium
• Cell-Culture Gels
• Media Supplements
• Attachment Factors
• Buffers
• Sodium
• Earle's Balanced Salt Solution
• Growth Factors
• Epidermal Growth Factor (EGF)
• Fibroblast Growth Factors (FGF)
• Acidic FGF
• Basic FGF
• Other Growth Factors
• Lectins
• Toxins
• Transport Factors
• Vitamins
• Water
• Manufacturers' Directory

Fermenters

• The Fermentation Process
• Enzyme Fermentation in the Organic Phase
• Cell Growth and Production
• Factors Affecting Specific Growth Rate
• Kinetic Models
• Optimal Conditions
• Recombinant Culture Kinetics
• Fermenter Design
• Kinetic Modeling of the Fermentation Process
• STF Batch Scale Up
• Power Number Method for STF Geometric Scale Up
• Continuous-Culture Scale Up
• Fermenter Manufacturers Directory

Bioreactors

• Aseptic Conditions
• Monoclonal Antibody Production
• mAB Production in Ascites Fluid
• Bioreactor Engineering and Design
• Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program
• Bioreactor Manufacturers Directory

Biomolecular Synthesizers

• DNA Synthesizers
• Manufacturers Directory
• Technology Overview
• Peptides by Solid-Phase Synthesis
• Manufacturers Directory

Index

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