This reference on protein purification has been thoroughly updated and rewritten where necessary. The core of the book is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. It is not comprehensive but does cover the major laboratory techniques and common sources of protein.

Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations.

It describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue.

Protein Purification Techniques also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. This book is designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

Contents

Abbreviations

1. Fusion protein purification methods, Stephen J. Brewer, Charles E. Glatz, and Charles Dickerson
2. Initial purification of inclusion bodies, Bernard N. Violand
3. Purification for crystallography, S. P. Wood and A. R. Coker
4. Protein purification from mammalian cell culture, Terry Cartwright
5. Protein purification from microbial cell culture, Peter R. Levison
6. Protein purification from milk, Richard Burr
7. Protein purification from animal tissue, Nigel M. Hooper
8. Protein purification from plant sources

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