Proteomics in Drug Research coverage of a wide range of technologies and areas of application.

Features:

• Includes proteomic technologies, such as protein biochips, protein-protein interactions, and proteome analysis in situ
• Details bioinformatics, Alzheimer's disease, neuroproteomics, plasma and T-cell proteomics, differential phosphoproteome analysis and biomarkers, as well as pharmacogenomics
• Provides a necessary perspective on current trends and developments

Contents

Administrative Optimization of Proteomics Networks for Drug Development

• Tasks and Aims of Administration.
• Networking.
• Evaluation of Biomarkers.
• A Network for Proteomics in Drug Development.
• Realization of Administrative Networking: the Brain Proteome Projects

Proteomic Data Standardization, Deposition and Exchange

• Protein Analysis Tools.
• Data Storage and Retrieval.
• The Proteome Standards Initiative.
• General Proteomics Standards (GPS).
• Mass Spectrometry.
• Molecular Interactions

Difference Gel Electrophoresis (DIGE): the Next Generation of Two-Dimensional Gel Electrophoresis for Clinical Research

• Difference Gel Electrophoresis: Next Generation of Protein Detection in 2-DE

Biological Mass Spectrometry: Basics and Drug Discovery Related Approaches

• Ionization Principles.
• Mass Spectrometric Instrumentation.
• Protein Identification Strategies.
• Quantitative Mass Spectrometry for Comparative and Functional Proteomics.
• Metabolic Labeling Approaches.
• Chemical Labeling Approaches.
• Quantitative MS for Deciphering Protein–Protein Interactions

Multidimensional Column Liquid Chromatography (LC) in Proteomics – Where Are We Now

• Why Do We Need MD-LC/MS Methods?
• Basic Aspects of Developing a MD-LC/MS Method.
• Applications of MD-LC Separation in Proteomics – a Brief Survey.
• Sample Clean-Up: Ways to Overcome the “Bottleneck” in Proteome Analysis

Peptidomics Technologies and Applications in Drug Research

• Peptides in Drug Research.
• Development of Peptidomics Technologies.
• Applications of Differential Display Peptidomics.
• Outlook

Protein Biochips in the Proteomic Field

• Technological Aspects.
• Applications of Protein Biochips.
• Contribution to Pharmaceutical Research and Development

Current Developments for the In Vitro Characterization of Protein Interactions

• The Model System: cAMP-Dependent Protein Kinase.
• Real-time Monitoring of Interactions Using SPR Biosensors.
• ITC in Drug Design.
• Fluorescence Polarization, a Tool for High-Throughput Screening.
• AlphaScreen as a Pharmaceutical Screening Tool

Molecular Networks in Morphologically Intact Cells and Tissue–Challenge for Biology and Drug Development

• A Metaphor of the Cell.
• Mapping Molecular Networks as Patterns: Theoretical Considerations.
• Imaging Cycler Robots.
• Formalization of Network Motifs as Geometric Objects.
• Gain of Functional Information: Perspectives for Drug Development

From Target to Lead Synthesis

• Materials and Methods

Differential Phosphoproteome Analysis in Medical Research

• Phosphoproteomics of Human Platelets.
• Identification of cAMP- and cGMP-Dependent Protein Kinase Substrates in Human Platelets.
• Identification of a New Therapeutic Target for Anti-Inflammatory Therapy by Analyzing Differences in the Phosphoproteome of Wild Type and Knock Out Mice.

Biomarker Discovery in Renal Cell Carcinoma Applying Proteome-Based Studies in Combination with Serology

• Rational Approaches Used for Biomarker Discovery.
• Advantages of Different Proteome-Based Technologies for the Identification of Biomarkers.
• Type of Biomarker.
• Proteome Analysis of Renal Cell Carcinoma Cell Lines and Biopsies.
• Validation of Differentially Expressed Proteins

Studies of Drug Resistance Using Organelle Proteomics

• Objectives and Experimental Design.
• Changes in Plasma Membrane and Nuclear Proteins in MCF-7 Cells Resistant to Mitoxantrone

Clinical Neuroproteomics of Human Body Fluids: CSF and Blood Assays for Early and Differential Diagnosis of Dementia

• Neurochemical Markers of Alzheimer’s Disease

Proteomics in Alzheimer’s Disease

• Proteomic Analysis.
• Proteins with Deranged Levels and Modifications in AD.
• Limitations

Cardiac Proteomics

• Heart Proteomics.
• Vessel Proteomics

Innovation Processes

• Innovation Process Criteria.
• The Concept.
• Market Attractiveness.
• Competitive Market Position.
• Competitive Technology Position.
• Strengthen the Fit.
• Reward.
• Risk.
• Innovation Process Deliverables for each Stage.
• Stage Gate-Like Process

Index

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