Proteomics Today discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.

• Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
• Explains the hows and whys of using mass spectrometry in proteomic analysis
• Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
• Comments on future challenges and how they may be addressed
• Includes sections on troubleshooting

Contents

Instrumentation and Developments

• Ionization Techniques for Macromolecules
• Examples on Analytical Solutions Based on FAB–MS
• Electrospray Ionization
• Matrix-Assisted Laser Desorption Ionization
• Ion Detection
• Types of Analyzers
• Hybrid Analyzers
• Tandem Mass Spectrometry
• Current MS Instrumentation in Proteome Analyses
• Current MS-Based Proteomics

Proteomics in Cancer Research

• Pancreatic Ductal Adenocarcinoma
• Proteomic Analysis of Human Breast Carcinoma
• Proteomic Profiling of Chemoresistant Cancer Cells
• Signal Pathway Profiling of Prostate Cancer
• Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding
• Activity-Based Protein Profiling
• Probing Protein Functions Using Chromophore-Assisted Laser Inactivation
• Role of Protein–Tyrosine Kinases

Current Strategies for Protein Quantification

• Global Internal Standard Technology
• Differential In-Gel Electrophoresis
• Quantification of Modified Proteins
• Comments and Considerations
• Other Approaches
• Emerging Role of Microfluidic Devices

Convential Isoelectric Focusing in Gel Matrices and Capillaries and Immobilized pH Gradients

• Conventional Isoelectric Focusing in Amphoteric Buffers
• Immobilized pH Gradients
• Capillary Isoelectric Focusing
• Separation of Peptides and Proteins by CZE in Isoelectric Buffers

Sodium Dodecyl Sulfate—Polyacrylamide Gel Electrophoresis

• SDS–Protein Complexes: a Refinement of the Model
• Theoretical Background of Mr Measurement by SDS–PAGE
• Methodology
• Gel Casting and Buffer Systems
• Blotting Procedures

Two-Dimensional Maps

• Some Basic Methodology Pertaining to 2D PAGE
• Prefractionation Tools in Proteome Analysis
• Multidimensional Chromatography Coupled to MS
• Protein Chips and Microarrays
• Nondenaturing Protein Maps
• Spot Matching in 2D Gels via Commercial Software

Index

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