Proteomics Sample Preparation is a major bottleneck in this fast growing technique within the molecular life sciences.

Proteomics Sample Preparation addresses the topic from three different angles, including:

• sample
• method
• aim

In Proteomics Sample Preparation a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.

Contents

General Aspects of Sample Preparation for Comprehensive Proteome Analysis

• The Need for Standards in Proteomics Sample Preparation
• General Aspects: Parameters which Influence the Sample Preparation Procedure

Proteomics: A Philosophical Perspective

• The Experiment as a Scientific Method and a Tool of Cognition
• The Experimaent as a Method of Cognition Within the Scope of Biology: The So-Called "Life Sciences"
• Protemics as a Cognition-Theoretical Challenge

Mass Spectrometry

• A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application
• Sample Preparation for the Application of MALDI Mass Spectrometry
• Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry
• Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr
• Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging

Electrophoresis

• Sample Preparation for Two-Dimensional Gel Electrophoresis
• Sample Preparation for Native Electrophoresis
• Sample Preparation for LC-MS/MS using Free Flow Electrophoresis
• Sample Preparation for Proteomics

Optical Methods

• High-Throughout Proteomics: Spinning Disc Interferometry (SDI)
• Optical Proteomics on Cell Arrays
• Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis
• Sample Preparation for Flow Cytometry

Chromatography

• Sample Preparation for HPLC-Based Proteome Analysis
• Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis

Structural Proteomics

• Exploring Protein-Ligand Interactions by Solution NMR
• Sample Preparation for Crystallography

Interaction Analysis

• Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP) Methods
• Exploring Membrane Proteomes

Post-Translational Modifications

• Sample Preparation for Phosphoproteome Analysis
• Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation

Species-Dependent Proteomics

• Sample Preparation and Data Processing in Plant Proteomics
• Sample Preparation for MudPIT with Bacterial Protein Samples
• Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi

The Human Proteosome

• Clinical Proteomics: Sample Preparation and Standardization
• Stem Cell Proteomics

Bioinformatics

• Bioinformatics Support for Mass Spectrometric Quality Control
• Use of Physio-Chemical Properties in Peptide and Protein Identification

Index

click here to see books of related interest