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Short Protocols in Neuroscience
Systems and Behavioral Methods
by Jacqueline N. Crawley

Short Protocols in Neuroscience provides a portable and streamlined at-the-bench resource for systems and behavioral methods from the acclaimed current protocols in neuroscience.

Contents

Neurophysiology

  • Fabrication of Patch Pipets
  • Whole-Cell Voltage Clamp Recording
  • Preparation of Hippocampal Brain Slices
  • Synaptic Plasticity in the Hippocampal Slice Preparation
  • Recording Synaptic Currents and Synaptic Potentials
  • Voltage Clamp Recordings from Xenopus Oocytes
  • Preparation and Maintenance of Organotypic Slice Cultures of CNS Tissue
  • Patch-Pipet Recording in Brain Slices
  • Single-Channel Recording
  • Acute Isolation of Neurons from the Mature Mammalian Central Nervous System
  • Patch-Clamp Recording from Neuronal Dendrites

Neurochemistry/Neuropharmacology

  • Scintillation Proximity Assay
  • Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid B-Peptides
  • Assessment of Cell Viability in Primary Neuronal Cultures
  • Measurement of NO and NO Synthase
  • Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
  • Overview of Microdialysis
  • Microdialysis in Rodents
  • Microdialysis in Nonhuman Primates
  • Detection and Quantification of Neurotransmitters in Dialysates
  • Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites
  • Uptake and Release of Neurotransmitters
  • Measurement of Chloride Movement in Neuronal Preparations
  • Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
  • Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
  • In Vivo Measurement of Blood-Brain Barrier Permeability

Behavioral Neuroscience

  • Assessment of Developmental Milestones in Rodents
  • Locomotor Behavior
  • Motor Coordination and Balance in Rodents
  • Basic Measures of Food Intake
  • Sexual and Reproductive Behaviors
  • Parental Bahavior in Rats and Mice
  • Applications of Experimental Stressors in Laboratory Rodents
  • Rodents Models of Depression: Forced Swimming and Tail Suspension Behavioral Despair Tests in Rats and Mice
  • Rodent Models of Depression: Learned Helplessness Induced in Mice
  • Animal Tests of Anxiety
  • Assessment of Spatial Memory Using the Radial Arm Maze and Morris Water Maze
  • Cued and Contextual Fear Conditioning in Mice
  • Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
  • Measurement of Startle Response, Prepulse Inhibition, and Habituation
  • Latent Inhibition

Preclinical Models of Neurologic and Psychiatric Disorders

  • Preclinical Models of Parkinson's Disease
  • Rodent Models of Global Cerebral Ischemia
  • Rodent Models of Focal Cerebral Ischemia
  • Inducing Photochemical Cortical Lesions in Rat Brain
  • Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
  • Models of Neuropathic Pain in the Rat
  • Dural Inflammation Model of Migraine Pain
  • Animal Models of Painful Diabetic Neuropathy: The STZ Rat Model
  • Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
  • Place Preference Test in Rodents
  • Intravenous Self-Administration of Ethanol in Mice
  • Preclinical Models to Evaluate Potential Pharmacotherapeutic Agents in Treating Alcoholism and Studying the Neuropharmacological Bases of Ethanol-Seeking Behaviors in Rats
  • Experimental Autoimmune Encephalomyelitis (EAE)
  • Models of Amyotrophic Lateral Sclerosis
  • Measurement of Panic-Like Responses Following Intravenous Infusion of Sodium Lactate in Panic-Prone Rats
  • Chemoconvulsant Model of Chronic Spontaneous Seizures

Index

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Short Protocols in Neuroscience
Systems and Behavioral Methods
by Jacqueline N. Crawley

2007 $128.00 + shipping
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