Systems and Behavioral Methods
by Jacqueline N. Crawley
Short Protocols in Neuroscience provides a portable and streamlined at-the-bench resource for systems and behavioral methods from the acclaimed current protocols in neuroscience.
Contents
Neurophysiology
- Fabrication of Patch Pipets
- Whole-Cell Voltage Clamp Recording
- Preparation of Hippocampal Brain Slices
- Synaptic Plasticity in the Hippocampal Slice Preparation
- Recording Synaptic Currents and Synaptic Potentials
- Voltage Clamp Recordings from Xenopus Oocytes
- Preparation and Maintenance of Organotypic Slice Cultures of CNS Tissue
- Patch-Pipet Recording in Brain Slices
- Single-Channel Recording
- Acute Isolation of Neurons from the Mature Mammalian Central Nervous System
- Patch-Clamp Recording from Neuronal Dendrites
Neurochemistry/Neuropharmacology
- Scintillation Proximity Assay
- Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid B-Peptides
- Assessment of Cell Viability in Primary Neuronal Cultures
- Measurement of NO and NO Synthase
- Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
- Overview of Microdialysis
- Microdialysis in Rodents
- Microdialysis in Nonhuman Primates
- Detection and Quantification of Neurotransmitters in Dialysates
- Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites
- Uptake and Release of Neurotransmitters
- Measurement of Chloride Movement in Neuronal Preparations
- Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
- Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
- In Vivo Measurement of Blood-Brain Barrier Permeability
Behavioral Neuroscience
- Assessment of Developmental Milestones in Rodents
- Locomotor Behavior
- Motor Coordination and Balance in Rodents
- Basic Measures of Food Intake
- Sexual and Reproductive Behaviors
- Parental Bahavior in Rats and Mice
- Applications of Experimental Stressors in Laboratory Rodents
- Rodents Models of Depression: Forced Swimming and Tail Suspension Behavioral Despair Tests in Rats and Mice
- Rodent Models of Depression: Learned Helplessness Induced in Mice
- Animal Tests of Anxiety
- Assessment of Spatial Memory Using the Radial Arm Maze and Morris Water Maze
- Cued and Contextual Fear Conditioning in Mice
- Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
- Measurement of Startle Response, Prepulse Inhibition, and Habituation
- Latent Inhibition
Preclinical Models of Neurologic and Psychiatric Disorders
- Preclinical Models of Parkinson's Disease
- Rodent Models of Global Cerebral Ischemia
- Rodent Models of Focal Cerebral Ischemia
- Inducing Photochemical Cortical Lesions in Rat Brain
- Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
- Models of Neuropathic Pain in the Rat
- Dural Inflammation Model of Migraine Pain
- Animal Models of Painful Diabetic Neuropathy: The STZ Rat Model
- Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
- Place Preference Test in Rodents
- Intravenous Self-Administration of Ethanol in Mice
- Preclinical Models to Evaluate Potential Pharmacotherapeutic Agents in Treating Alcoholism and Studying the Neuropharmacological Bases of Ethanol-Seeking Behaviors in Rats
- Experimental Autoimmune Encephalomyelitis (EAE)
- Models of Amyotrophic Lateral Sclerosis
- Measurement of Panic-Like Responses Following Intravenous Infusion of Sodium Lactate in Panic-Prone Rats
- Chemoconvulsant Model of Chronic Spontaneous Seizures
Index
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