Neuroscience Book from C.H.I.P.S.

Short Protocols in Neuroscience
Cellular and Molecular Methods
edited by Charles Gerfen

Short Protocols in Neuroscience is an easy-to-use compendium of cellular and molecular methods. It provides a portable and streamlined resource, covering:

  • methods for analysis of neural cell structure and function
  • isolation and culture of neural cells
  • characterization of neural cell gene expression
  • and more


Neuroanatomical Methods

  • Basic Neuroanatomical Methods
  • Immunohistochemical Localization of Proteins in the Nervous System
  • Hybridization Histochemistry of Neural Transcripts
  • Receptor Binding Techniques
  • Intracellular and Juxtacellular Staining with Biocytin


  • Theory and Application of Fluorescence Microscopy
  • Basic Confocal Microscopy
  • Imaging Nervous System Activity
  • Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
  • Using FM1-43 and Other Derivatives to Investigate Neuronal Function
  • Multidisciplinary Approaches to Characterizing Synaptic Vesicle Proteins

Cellular and Developmental Neuroscience

  • Culture of Neuroepithelial Stem Cells
  • Differentiation of Embryonic Stem Cells
  • Quantitative Analysis of In Vivo Cell Proliferation
  • Long-Term Culture of Hippocampal Neurons
  • Culture of Substantia Nigra Neurons
  • Isolation and Purification of Primary Rodent Astrocytes
  • Experimental Transplantation in the Embryonic, Neonatal, and Adult Mammalian Brain

Gene Cloning, Expression, Metagenesis

  • PCR Cloning of Neural Gene Products
  • Interaction Trap/Two Hybrid System to Identify Interacting Proteins
  • cDNA Library Construction from Single Cells
  • Overview of Neural Gene Expression
  • Selection of Transfected Mammalian Cells
  • Protein Expression in the Drosophila Schneider 2 Cell System
  • Protein Expression in the Baculovirus System
  • Gene Transfer into Neural Cells in Vitro Using Adenoviral Vectors
  • Gene Transfer into Rat Brain Using Adenoviral Vectors
  • Production of High-Titer Lentivirus Vectors
  • Production of Recombinant Adeno-Associated Viral Vectors and Use In Vitro and In Vivo Administration
  • Alphavirus-Mediated Gene Transfer into Neurons
  • Overview of Gene Delivery into Cells Using HSV-1 Vectors
  • Generation of High-Titer Defective HSV-1 Vectors
  • Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
  • Overview of Nucleic Acid Arrays
  • Preparation of mRNA for Expression Monitoring
  • Gene Expression Analysis Using cDNA Microarrays
  • Overview of Gene Targeting by Homologous Recombination

Molecular Neuroscience

  • Analysis of RNA in Northern and Slit-Blot Hybridization
  • RNA Analysis by Nuclease Protection
  • Analysis of mRNA Populations from Single Live and Fixed Cells from the Central Nervous system
  • Overview of RNAi
  • Production of Antipeptide Antisera
  • Production of Antisera Using Fusion Proteins
  • Immunoblotting and Immunodetection
  • The Use of Phage Display in Neurobiology
  • Using Phage Display in Neurobiology
  • Detection of Protein Phosphorylation in Tissues and Cells
  • Overview of Membrane Protein Solubilization
  • Immunoprecipitation
  • Coimmunoprecipitation
  • FRET
  • An Overview on the Generation of BAC Transgenic Mice for Neuroscience Research
  • Modification of Bacterial Artificial Chromosomes (BACs) and Preparation of Intact BAC DNA for Generation of Transgenic Mice


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Short Protocols in Neuroscience
Cellular and Molecular Methods
edited by Charles Gerfen

2006 • 592 pages • $128.00 + shipping
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