Short Protocols in Pharmacology and Drug Discovery provides condensed descriptions of more than 350 methods compiled from the successful title Current Protocols in Pharmacology.
This book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of pharmaceutical research, and especially stresses drug discovery techniques.
Partial Contents
1. Introduction to Drug Discovery and Development
- Overview of drug discovery and development
2. Receptor Characterization
- Receptors as drug targets
- Receptor theory and function
- Practical aspects of radioligand binding
- Measurement of allosteric interactions at G protein-coupled receptors using radioligand binding
- Characterization of opioid receptor binding and function
- Characterization of GABA, receptor binding
3. Enzyme Assays
- Overview of signal transduction
- Adenylyl and guanylyl cyclase assays
- Assays for cyclic nucleotide-specific phosphodiesterases
- Phosphoinositide turnover
- Nitric oxide synthase assays
- HIV protease assays
- Topoisomerase assays
- Cytochrome P450 assays
4. Isolated Tissues
- Overview of length-tension relationships in isolated tissue
- Isolated cardiac muscle assays
- Isolated blood vessel assays
- α-adrenoceptor assays
- β-adrenoceptor assays
- P1 (adenosine) purinoceptor assays
- Opioid receptor assays
- Muscarinic receptor assays
- Histamine receptor assays
- Prostanoid receptor assays
- cysteinyl leukotriene receptor assays
- 5-hydroxytryptamine receptor assays
5. Animal Models of Disease
6. Pharmacokinetics
7. Drug Discovery Technologies
8. Safety Pharmacology/Tocicology
Appendices
- Reagents and solutions
- Nomenclature and usage
- Standard techniques
- Selected suppliers of reagents and equipment
Index
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