Spectroscopy for the Biological Sciences presents the physical principles underlying spectroscopy with a minimum of mathematics.
Examples and applications to the biological sciences are included.
Contents:
- Fundamentals of Spectroscopy
- Introduction
- Quantum Mechanics
- Particle in a Box
- Properties of Waves
- References
- Problems
- X-ray Crystallography
- Introduction
- Scattering of X-rays by a Crystal
- Structure Determination
- Neutron Diffraction
- Nucleic Acid Structure
- Protein Structure
- Enzyme Catalysis
- References
- Problems
- Electronic Structure
- Introduction
- Absorption Spectra
- Ultraviolet Spectra of Proteins
- Nucleic Acid Spectra
- Prosthetic Groups
- Difference Spectroscopy
- X-ray Absorption Spectroscopy
- Fluorescence and Phosphorescence
- RecBCD: Helicase Activity Monitored by Fluorescence
- Fluorescence Energy Transfer: A Molecular Ruler
- Application of Energy Transfer to Biological Systems
- Dihydrofolate Reductase
- References
- Problems
- Circular Dichroism, Optical Rotary Dispersion, and Fluorescence Polarization
- Introduction
- Optical Rotary Dispersion
- Circular Dichroism
- Optical Rotary Dispersion and Circular Dichroism of Proteins
- Optical Rotary Dispersion and Circular Dichroism of Nucleic Acids
- Small Molecule Binding to DNA
- Protein Folding
- Interaction of DNA with Zinc Finger Proteins
- Fluorescence Polarization
- Integration of HIV Genome into Host Genome
- a-ketoglutarate Dehyrogenase100
- References
- Problems
- Vibrations in Macromolecules
- Introduction
- Infrared Spectroscopy
- Raman Spectroscopy
- Structure Determination with Vibrational Spectroscopy
- Resonance Raman Spectroscopy119
- Structure of Enzyme-Substrate Complexes
- References
- Problems
- Principles of Nuclear Magnetic Resonance and Electron SpinResonance
- Introduction
- NMR Spectrometers
- Chemical Shifts
- Spin-spin Splitting
- Relaxation Times
- Multi-dimensional NMR
- Magnetic Resonance Imaging
- Electron Spin Resonance
- References
- Problems
- Applications of Magnetic Resonance in Biology
- Introduction
- Regulation of DNA Transcription
- Protein-DNA Interactions
- Dynamics of Protein Folding
- RNA Folding
- Lactose Permease
- Conclusion
- References
- Mass Spectrometry
- Introduction
- Mass Analysis
- Tandem Mass Spectrometry
- Ion Detectors
- Ionization of the Sample
- Sample Preparation/Analysis
- Proteins and Peptides
- Protein Folding
- Other Biomolecules
- References
- Problems
Appendix 1. Useful Constants and Conversion Factors
Appendix 2. Structures of the Common Amino Acids at Neutral pH
Appendix 3. Common Nucleic Acid Components
Index
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