by Dan Luo and W. Mark Saltzman

DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function.

Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest.

Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Contents

1. Supramolecular Assemblies of DNA Delivery Systems
2. Engineering the Surface Properties of Synthetic Gene Delivery Vectors
3. Histidine Containing Peptides and Polypeptides as Nucleic Acid Vectors
4. Intracellular Targets for DNA Delivery: Nuclei and Mitochondria
5. Mannose Receptor-Mediated Gene Delivery into Antigen Presenting
6. Electro-Gene Transfer: A New Approach for Muscle Gene Delivery
7. Tumor-Targeted Gene Transfer with DNA Polyplexes
8. Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery
9. Sonoporation: Mechanical DNA Delivery by Ultrasonic Cavitation

Index

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