Techniques for Molecular Biology is presented in the form of profiles, and explains simply the theoretical principles of techniques of molecular biology.

This edition contains new illustrations and describes numerous techniques of genomic studies that have been develo0ped recently in laboratories.

Contents

Definition

1. Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein
2. Parameters of Description of a Genome
3. Sequencing of Entire Genomes

Vectors and Cloning

4. Restriction Enzymes
5. Electrophoresis of Nucleic Acids
6. Description of a Plasmid and a Phagemid
7. Description of a Bacteriophage and a Cosmid
8. Description of a YAC and Other Large Vectors
9. Molecular Cloning
10. Genetic Transformation of Bacteria and Yeasts

Labelling of Nucleic Acids and Hybridization

11. DNA Labelling
12. Molecular Hybridization
13. In situ Hybridization of mRNA

DNA Libraries and Screening

14. Construction of a Genomic DNA Library
15. Construction of a cDNA Library
16. Screening of a Library
17. Differential Display: Subtractive Libraries, AFLP-cDNA
18. Differential Display RT-PCR: mRNA Screening
19. Differential Display SSH: Suppression Subtractive Hybridization
20. Differential Screening by RDA: Representational Difference Analysis
21. EST: Expressed Sequence Tags
22. DNA Microarrays: DNA Microchips, cDNA Filters

Characterization of a Gene

23. DNA Sequencing
24. PCR: Polymerase Chain Reaction
25. RACE: Rapid Amplification of cDNA Ends
26. Genome Walking by PCR
27. RT-PCR: Reverse Transcriptase PCR
28. In vitro Transcription
29. Determination of Transcription Initiation Sit
30. Functional Analysis of Promoters
31. Gel Retarda-tion
32. DNAse I Footprinting

Genetic Transformation of Euaryotes

33. Genetic Transformation of Plants by Agrobacterium tumefaciens
34. Direct Gene Transfer into Plant Protoplasts
35. Direct Gene Transfer by Biolistics
36. Genetic Transformation of Animal Cells
37. Cloning of Animals
38. Transient Expression

Analysis of Gene Function

39. Recombinant Proteins
40. Baculoviruses of Insects, Vectors of Expression of Foreign Genes
41. Double Hybrid System
42. Directed Mutagenesis
43. Mutation Complementation in Yeast
44. Knock-out of Genes
45. Gene Tagging
46. RNA Interference

Polymorphism of a Genome

47. Molecular Markers
48. Genetic and Physical Maps
49. PFGE: Pulse Field Gel Electrophoresis
50. RFLP: Restriction Fragment Length Polymorphism
51. RAPD: Random Amplified Polymorphic DNA
52. AFLP: Amplified Fragment Length Polymorphism
53. Retromarkers
54. SSCP: Single Strand Conformation Polymorphism
55. DGGE: Denaturing Gel Gradient Electrophoresis
56. SNP: Single Nucleotide Polymorphism
57. Simple Sequence Repeats (SSR): Microsatellites

List of Contributors

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