Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications.

Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc.

• Underscores the advantages of using TLC for locating and analyzing bioactive compounds from plant sources
• Explains the benefits of using TLC over more expensive and complex chromatographic techniques
• Reviews the techniques, materials, instrumentation, and procedures specific to the analysis and isolation of each compound class
• Discusses methods for online derivatization, detection, identification, and quantification
• Includes up-to-date tables, figures, and references throughout the text

Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components.

Contents

Part 1

1. Overview of the Field of TLC in Phytochemistry and the Structure of the Book
2. Plant Materials in Modern Pharmacy and Methods of Their Investigations
3. Medicines and Dietary Supplements Produced from Plants
4. Primary and Secondary Metabolites and Their Biological Activity
5. Plant Chemosystematics
6. Sorbents and Precoated Layers for the Analysis and Isolation of Primary and Secondary Metabolites
7. Chambers, Sample Application, and Chromatogram Development
8. Derivatization, Detection (Quantification), and Identification of Compounds Online
9. Biodetection and Determination of Biological Activity of Natural Compounds
10. Forced-Flow Planar Layer Liquid Chromatographic Techniques for the Separation and Isolation of Natural Substances

Part 2

11. Primary Metabolites
12. TLC of Carbohydrates
13. TLC of Lipids
14. Amino Acids
15. Secondary Metabolites—Shickimic Acid Derivatives
16. Sample Preparation and TLC Analysis of Phenolic Acids
17. Application of TLC in the Isolation and Analysis of Coumarins
18. Application of TLC in the Isolation and Analysis of Flavonoids
19. TLC of Lignans
20. Secondary Metabolites—Isoprenoids
21. TLC of Mono- and Sesquiterpenes
22. TLC of Diterpenes
23. TLC of Triterpenes (Including Saponins)
24. TLC of Carotenoids
25. TLC of Sterols, Steroids, and Related Triterpenoids
26. TLC of Iridoids
27. Secondary Metabolites—Amino Acid Derivatives
28. TLC of Indole Alkaloids
29. TLC of Isoquinoline Alkaloids
30. TLC of Tropane Alkaloids
31. TLC of Alkaloids from the Other Biosynthetic Groups
32. Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)
33. Polyacetylenes: Distribution in Higher Plants, Pharmacological Effects, and Analysis
34. Quinone Derivatives in Plant Extracts

Index

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